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Abstract <p>The medicinal plant <i>Saraca asoca</i> harbors an endophytic fungal strain, which was identified as <i>Pestalotiopsis</i> sp. L12 by morphological and molecular analyses. It exhibited maximum amylase and cellulase production at 28°C after 72 h, releasing 41.66 µg of maltose and 35.66 µg of glucose. The crude extracts of isolate L12 showed antibacterial activity against such important pathogens as <i>K. pneumoniae</i>, <i>S. aureus</i>, and <i>E.&#xa0;coli</i> in addition to antioxidant properties. The effective concentration (EC50) of the crude extract for antioxidant activity against ABTS<sup>+</sup> radical was 64 µg/mL. Furthermore, the GC-MS and HPLC-ESI-MS/MS analyses of the crude extracts identified six and seven compounds, respectively. Notably, one of the compounds, 6,9-dimethyltetradecan, is known for its antibacterial and antioxidant properties. Oleic acid and theophylline identified through mass fragmentation in HPLC-ESI-MS/MS analysis have also been well-recognized for antibacterial and antioxidant activities.</p>

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Metabolites Profiling, Bioactivity Assessment and Extracellular Enzymes Estimation of Pestalotiopsis sp. L12, an Endophytic Fungus Recovered from Saraca asoca (Roxb.) Willd. Leaves

  • 期刊论文

  • 发表日期：

    2025年11月17日

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  • J. H. Nishad,
  • S. Vishwakarma,
  • A. Singh,
  • M. Yadav,
  • R. Bharti,
  • R. N. Kharwar

摘要

Abstract

The medicinal plant Saraca asoca harbors an endophytic fungal strain, which was identified as Pestalotiopsis sp. L12 by morphological and molecular analyses. It exhibited maximum amylase and cellulase production at 28°C after 72 h, releasing 41.66 µg of maltose and 35.66 µg of glucose. The crude extracts of isolate L12 showed antibacterial activity against such important pathogens as K. pneumoniae, S. aureus, and E. coli in addition to antioxidant properties. The effective concentration (EC50) of the crude extract for antioxidant activity against ABTS+ radical was 64 µg/mL. Furthermore, the GC-MS and HPLC-ESI-MS/MS analyses of the crude extracts identified six and seven compounds, respectively. Notably, one of the compounds, 6,9-dimethyltetradecan, is known for its antibacterial and antioxidant properties. Oleic acid and theophylline identified through mass fragmentation in HPLC-ESI-MS/MS analysis have also been well-recognized for antibacterial and antioxidant activities.