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<b>Abstract</b>— <p>The goal of the present work was to characterize the relationship between the persistence profile and the ability to produce iron-binding compounds in intestinal isolates of <i>E. coli</i> and <i>K. pneumoniae</i> with the genetic determinants <i>iucBC</i> and <i>clbBN</i>, both at the phenotypic and genetic levels. The use of designed primers and an unified multiplex-PCR algorithm revealed a high occurrence frequency (44.0—80.0%) of strains with genetic determinants <i>clbBN</i> and <i>iucBC</i> encoding the synthesis of aerobactin and colibactin in the population of opportunistic enterobacteria. The presence of the <i>clbBN</i>/<i>iucBC</i> genes in the genetic apparatus of enterobacteria was found to result in a manifest ability to produce iron-binding compounds and a wide range of persistent characteristics, including antilysozyme, anticarnosine, and antipeptide activities with respect to TNFα and anti-immunoglobulin activity with respect to IgM/IgG and biofilm formation. Comparison of the genomes of sequenced <i>clbBN</i><sup><i>+</i></sup><i>iucBC</i><sup><i>+</i></sup> strains with those of <i>clbBN</i><sup><Emphasis Type="BoldItalic">−</Emphasis></sup><i>iucBC</i><sup><Emphasis Type="BoldItalic">−</Emphasis></sup> strains revealed the presence of genes responsible for aerobactin biosynthesis and transport, a complete set of <i>pks</i> island genes, and well-known homologues of the lysozyme inhibitor determinants <i>Ivy</i> and <i>MliC, EspP</i> (<i>E. coli</i> M-17) and <i>PliC, LprI</i> (<i>K. pneumoniae</i> ICIS-278 and <i>K. pneumoniae</i> ICIS-277). Therefore, the strains of <i>E. coli</i> and <i>K.&#xa0;pneumoniae</i> that possess the genetic determinants of iron chelators <i>clbBN</i> and <i>iucBC</i> meet the genotypic and phenotypic criteria of siderophore production and protease activity against the host antimicrobial factors. This enables utilizing these strains as markers of the pathogenic and persistence potential of opportunistic enterobacteria.</p>

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Persistence Profiles of Escherichia coli and Klebsiella pneumoniae Strains with Genetic Determinants of Iron Chelators

  • 期刊论文

  • 发表日期：

    2025年4月1日

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  • O. V. Bukharin,
  • E. V. Ivanova,
  • I. A. Zdvizhkova,
  • N. B. Perunova

摘要

Abstract—

The goal of the present work was to characterize the relationship between the persistence profile and the ability to produce iron-binding compounds in intestinal isolates of E. coli and K. pneumoniae with the genetic determinants iucBC and clbBN, both at the phenotypic and genetic levels. The use of designed primers and an unified multiplex-PCR algorithm revealed a high occurrence frequency (44.0—80.0%) of strains with genetic determinants clbBN and iucBC encoding the synthesis of aerobactin and colibactin in the population of opportunistic enterobacteria. The presence of the clbBN/iucBC genes in the genetic apparatus of enterobacteria was found to result in a manifest ability to produce iron-binding compounds and a wide range of persistent characteristics, including antilysozyme, anticarnosine, and antipeptide activities with respect to TNFα and anti-immunoglobulin activity with respect to IgM/IgG and biofilm formation. Comparison of the genomes of sequenced clbBN+iucBC+ strains with those of clbBN−iucBC− strains revealed the presence of genes responsible for aerobactin biosynthesis and transport, a complete set of pks island genes, and well-known homologues of the lysozyme inhibitor determinants Ivy and MliC, EspP (E. coli M-17) and PliC, LprI (K. pneumoniae ICIS-278 and K. pneumoniae ICIS-277). Therefore, the strains of E. coli and K. pneumoniae that possess the genetic determinants of iron chelators clbBN and iucBC meet the genotypic and phenotypic criteria of siderophore production and protease activity against the host antimicrobial factors. This enables utilizing these strains as markers of the pathogenic and persistence potential of opportunistic enterobacteria.