Scholarlink

Abstract <p>A new sulfate-reducing strain SRJS8<sup>T</sup> with a high ability to biodegrade petroleum hydrocarbons was isolated from the bottom sediments in the northern part of the Sea of Japan. The cells were gram-positive, spore-forming, singlemotile rods 0.4–0.5 × 2.0–5.0 µm in size. Strain SRJS8<sup>T</sup> grew at the temperature range&#xa0;from 6 to 30°C (optimum, 25°C), pH 6.3–7.7 (optimum, 7.3), and NaCl concentrations ranging from 0 to 20 g/L (optimum, 2 g/L). Strain SRJS8<sup>T</sup> utilized butanol, glycerol, methanol, ethanol, butyrate, lactate, pyruvate, formate, yeast extract, H<sub>2</sub>/CO<sub>2</sub>, and crude oil as electron donors and carbon sources in the presence of sulfate. SRJS8<sup>T</sup> utilized sulfate, sulfite, thiosulfate, elemental sulfur, fumarate, and Fe(III) as electron acceptors in the presence of lactate. The closest relative of the SRJS8<sup>T</sup> was <i>Desulfosporosinus lacus</i> STP12<sup>T</sup> (similarity, 98.49%). The complete genome of the strain SRJS8<sup>T</sup> (5.43 Mb) was sequenced. DNA–DNA homology of strain SRJS8<sup>T</sup> and <i>D. lacus</i> was 57.4%, and the ANI value was 93.69%. The genomic DNA G+C content was 42.08%. In accordance with the obtained data, SRJS8<sup>T</sup> was assigned to a novel species within the genus <i>Desulfosporosinus</i>, for which the name <i>Desulfosporosinus shakirovi</i> sp. nov. is proposed. The type strain is SRJS8<sup>T</sup> (=VKM B-3489<sup>T</sup>, =JCM 39 189<sup>T</sup>).</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Desulfosporosinus shakirovi sp. nov., a Sulfate-Reducing Bacterium Capable of Degrading Petroleum Hydrocarbons

  • 期刊论文

  • 发表日期：

    2025年6月14日

查看全文
  • A. I. Eskova,
  • Ya. V. Ryzhmanova,
  • V. E. Trubitsyn,
  • N. S. Polonik,
  • A. L. Ponomareva,
  • V. A. Shcherbakova

摘要

Abstract

A new sulfate-reducing strain SRJS8T with a high ability to biodegrade petroleum hydrocarbons was isolated from the bottom sediments in the northern part of the Sea of Japan. The cells were gram-positive, spore-forming, singlemotile rods 0.4–0.5 × 2.0–5.0 µm in size. Strain SRJS8T grew at the temperature range from 6 to 30°C (optimum, 25°C), pH 6.3–7.7 (optimum, 7.3), and NaCl concentrations ranging from 0 to 20 g/L (optimum, 2 g/L). Strain SRJS8T utilized butanol, glycerol, methanol, ethanol, butyrate, lactate, pyruvate, formate, yeast extract, H2/CO2, and crude oil as electron donors and carbon sources in the presence of sulfate. SRJS8T utilized sulfate, sulfite, thiosulfate, elemental sulfur, fumarate, and Fe(III) as electron acceptors in the presence of lactate. The closest relative of the SRJS8T was Desulfosporosinus lacus STP12T (similarity, 98.49%). The complete genome of the strain SRJS8T (5.43 Mb) was sequenced. DNA–DNA homology of strain SRJS8T and D. lacus was 57.4%, and the ANI value was 93.69%. The genomic DNA G+C content was 42.08%. In accordance with the obtained data, SRJS8T was assigned to a novel species within the genus Desulfosporosinus, for which the name Desulfosporosinus shakirovi sp. nov. is proposed. The type strain is SRJS8T (=VKM B-3489T, =JCM 39 189T).