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Abstract <p>It is widely recognized that successful proliferation of mammaliancells requires a complex of growth factors. When culturing cellsin vitro, the standard supplement is fetal bovine serum (FBS). However,numerous studies demonstrate that human platelet lysate (hPL) hascomparable efficacy in stimulating cell proliferation. Plateletscontain protein- and growth factor-rich granules whose secretionpromotes wound healing and tumor growth. Here, we investigated theeffect of hPL on the proliferation of mammalian cells. The study employedstandard culture methods for adherent human cell lines HEK293, MCF-7,SiHa, Ea.hy926, SH-SY5Y, OKP-GS, suspension human cell line K562,and adherent mouse cell lines EMT-6 and B16-F10. As a culture mediumsupplement, FBS was compared with hPL derived from a pool of humanplatelet concentrates from 10 healthy donors. hPL represents a solutionobtained by the triple freeze-thaw technique from a pooled plateletconcentrate, followed by filtration and addition of 0.1 U/mL heparin.Cell viability and proliferation were assessed by flow cytometryand the MTT assay. Cell morphology was analyzed using phase contrast microscopy.hPL, comparably to FBS, stimulated the growth of almost all culturesunder study, except for the human endothelial line Ea.hy926, forwhich ambiguous results were obtained. Almost all adherent celllines demonstrated a fibroblast-like growth characterized by reducedcell–cell contacts. The most significant differences in morphologywere observed in SH-SY5Y and Ea.hy926 cell lines, which became proneto forming long axons. Thus, platelet lysate successfully supportsmammalian cell proliferation; however, it also affects cell morphology,preventing the formation of an epithelial-like monolayer.</p>

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Effect of Platelet Lysate on Mammalian Cell Proliferation

  • 期刊论文

  • 发表日期：

    2026年2月26日

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  • I. S. Kolesnikova,
  • V. E. Belchikov,
  • A. A. Mishukov,
  • M. A. Panteleev,
  • P. E. Trakhtman,
  • A. N. Sveshnikova

摘要

Abstract

It is widely recognized that successful proliferation of mammaliancells requires a complex of growth factors. When culturing cellsin vitro, the standard supplement is fetal bovine serum (FBS). However,numerous studies demonstrate that human platelet lysate (hPL) hascomparable efficacy in stimulating cell proliferation. Plateletscontain protein- and growth factor-rich granules whose secretionpromotes wound healing and tumor growth. Here, we investigated theeffect of hPL on the proliferation of mammalian cells. The study employedstandard culture methods for adherent human cell lines HEK293, MCF-7,SiHa, Ea.hy926, SH-SY5Y, OKP-GS, suspension human cell line K562,and adherent mouse cell lines EMT-6 and B16-F10. As a culture mediumsupplement, FBS was compared with hPL derived from a pool of humanplatelet concentrates from 10 healthy donors. hPL represents a solutionobtained by the triple freeze-thaw technique from a pooled plateletconcentrate, followed by filtration and addition of 0.1 U/mL heparin.Cell viability and proliferation were assessed by flow cytometryand the MTT assay. Cell morphology was analyzed using phase contrast microscopy.hPL, comparably to FBS, stimulated the growth of almost all culturesunder study, except for the human endothelial line Ea.hy926, forwhich ambiguous results were obtained. Almost all adherent celllines demonstrated a fibroblast-like growth characterized by reducedcell–cell contacts. The most significant differences in morphologywere observed in SH-SY5Y and Ea.hy926 cell lines, which became proneto forming long axons. Thus, platelet lysate successfully supportsmammalian cell proliferation; however, it also affects cell morphology,preventing the formation of an epithelial-like monolayer.