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Abstract <p>Barrier properties of the intestinal epithelium play an importantrole in maintaining homeostasis, but become vulnerable under oxygendeficiency. This study investigated the effects of hypobaric hypoxia (simulatingan ascent to altitudes of 3000 and 6000 m) on the morphologicalparameters and barrier function of the jejunum and colon in rats.Experiments were carried out using a hypobaric chamber and includedhistological analysis, electrophysiological measurements in an Ussingchamber, as well as assessing tight junction protein levels (claudin-1and -2) by Western blotting and their localization by immunohistochemistry.It was found that hypobaric hypoxia induces time- and intestinalsegment-specific alterations in morphometric parameters, barriercharacteristics, and claudin levels. In the jejunum, 3 h after hypoxicexposure, the structure of the intestinal villi was disrupted andclaudin-2 levels decreased; in the colon, crypt depth was altered,claudin-1 levels decreased, and transepithelial resistance dropped.Twenty-four hours after hypoxic exposure (3000 m), jejunal functionwas partially recovered, whereas after exposure to a more severehypoxia (6000 m), no adaptation was observed in the colon. The obtaineddata emphasize the high sensitivity of the intestinal barrier tohypoxic stress and its differential segment-dependent responsiveness.The results may be useful in developing preventive strategies aimedat protecting the intestinal barrier under hypoxic conditions, includinghigh-altitude expeditions or pathological states associated withimpaired oxygen supply.</p>

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Changes in Barrier Properties of the Rat Jejunum and Colon after Hypobaric Hypoxia

  • 期刊论文

  • 发表日期：

    2025年7月1日

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  • A. A. Fedorova,
  • D. D. Ganke,
  • A. E. Bikmurzina,
  • V. V. Kravtsova,
  • I. I. Krivoi,
  • A. G. Markov

摘要

Abstract

Barrier properties of the intestinal epithelium play an importantrole in maintaining homeostasis, but become vulnerable under oxygendeficiency. This study investigated the effects of hypobaric hypoxia (simulatingan ascent to altitudes of 3000 and 6000 m) on the morphologicalparameters and barrier function of the jejunum and colon in rats.Experiments were carried out using a hypobaric chamber and includedhistological analysis, electrophysiological measurements in an Ussingchamber, as well as assessing tight junction protein levels (claudin-1and -2) by Western blotting and their localization by immunohistochemistry.It was found that hypobaric hypoxia induces time- and intestinalsegment-specific alterations in morphometric parameters, barriercharacteristics, and claudin levels. In the jejunum, 3 h after hypoxicexposure, the structure of the intestinal villi was disrupted andclaudin-2 levels decreased; in the colon, crypt depth was altered,claudin-1 levels decreased, and transepithelial resistance dropped.Twenty-four hours after hypoxic exposure (3000 m), jejunal functionwas partially recovered, whereas after exposure to a more severehypoxia (6000 m), no adaptation was observed in the colon. The obtaineddata emphasize the high sensitivity of the intestinal barrier tohypoxic stress and its differential segment-dependent responsiveness.The results may be useful in developing preventive strategies aimedat protecting the intestinal barrier under hypoxic conditions, includinghigh-altitude expeditions or pathological states associated withimpaired oxygen supply.