Scholarlink

Abstract <p>While the classical function of granulocyte colony-stimulatingfactor (G-CSF) is the regulation of neutrophil growth and differentiation,this cytokine also plays an important role in folliculo- and embryogenesis,embryo implantation and trophoblast invasion, thus arousing significantinterest in its application in assisted reproductive technology(ART) programs. It has been established that intrauterine G-CSFadministration in ART protocols for patients with chronic endometritisand recurrent implantation failure increases pregnancy rates; however,the pathogenetic mechanisms of its action require further investigation.We have developed a liposomal form of G-CSF, characterized by ahigher bioavailability upon intrauterine administration comparedto an aqueous G-CSF solution. The study involved 22 infertile patientswith a thin endometrium and repeated ART failures, who receivedtherapy for chronic endometritis via intrauterine administrationof the liposomal G-CSF form twice a week for 6 months. Using real-timePCR, the expression of <i>TVP23A</i>, <i>IL10</i>, and <i>TGF-β1</i> genesin endometrial biopsies was assessed before and after 6 months oftreatment with the liposomal G-CSF form. This therapy significantlyreduced the expression of the <i>TVP23A</i> gene(<i>p</i> &lt; 0.001) and increasedthe expression of <i>IL10</i> (<i>p</i> &lt; 0.001) and TGF-β1 (<i>p</i> &lt; 0.001) genes in the endometrium.A significant (<i>p</i> &lt; 0.001)increase in endometrial thickness (M-echo) from 5.6 ± 0.6 to 6.8± 0.8 mm was observed. The obtained data demonstrate that therapyfor chronic endometritis via intrauterine administration of the liposomalG-CSF form for 6 months normalizes the expression of some genesassociated with chronic endometrial inflammation.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Effect of Granulocyte Colony-Stimulating Factor Liposomal Form on the Expression of Genes Associated with Chronic Endometritis

  • 期刊论文

  • 发表日期：

    2025年7月1日

查看全文
  • J. R. Ryzhov,
  • K. V. Obedkova,
  • V. V. Khalenko,
  • A. M. Gzgzyan,
  • N. I. Tapilskaya

摘要

Abstract

While the classical function of granulocyte colony-stimulatingfactor (G-CSF) is the regulation of neutrophil growth and differentiation,this cytokine also plays an important role in folliculo- and embryogenesis,embryo implantation and trophoblast invasion, thus arousing significantinterest in its application in assisted reproductive technology(ART) programs. It has been established that intrauterine G-CSFadministration in ART protocols for patients with chronic endometritisand recurrent implantation failure increases pregnancy rates; however,the pathogenetic mechanisms of its action require further investigation.We have developed a liposomal form of G-CSF, characterized by ahigher bioavailability upon intrauterine administration comparedto an aqueous G-CSF solution. The study involved 22 infertile patientswith a thin endometrium and repeated ART failures, who receivedtherapy for chronic endometritis via intrauterine administrationof the liposomal G-CSF form twice a week for 6 months. Using real-timePCR, the expression of TVP23A, IL10, and TGF-β1 genesin endometrial biopsies was assessed before and after 6 months oftreatment with the liposomal G-CSF form. This therapy significantlyreduced the expression of the TVP23A gene(p < 0.001) and increasedthe expression of IL10 (p < 0.001) and TGF-β1 (p < 0.001) genes in the endometrium.A significant (p < 0.001)increase in endometrial thickness (M-echo) from 5.6 ± 0.6 to 6.8± 0.8 mm was observed. The obtained data demonstrate that therapyfor chronic endometritis via intrauterine administration of the liposomalG-CSF form for 6 months normalizes the expression of some genesassociated with chronic endometrial inflammation.