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Abstract <p>A growing amount of research is showing that miRNA plays acritical role in the pathophysiology of sepsis. Nevertheless, itis still unclear how miR-223-3p regulates sepsis. The purpose ofthis study is to look into the relationship and underlying mechanismbetween sepsis and miR-223-3p. We used qRT-PCR to assess RNA expression.Cell apoptosis was detected employing flow cytometry. The relationship betweenmiR-223-3p and its targeted protein was investigated utilizing luciferasereporter assays. Protein expression was analyzed through westernblot and immunofluorescence, while cell viability was determinedusing the CCK-8 assay. The results showed that miR-223-3p was significantlyup-regulated in septic patient serum and LPS-induced RAW264.7 cells,while the expression of FBXW7 showed the opposite pattern. Knockdownof miR-223-3p resulted in a decrease in Bax and cleaved caspase-3 expression,as well as an increase in Bcl-2 expression in LPS-induced RAW264.7cells. Conversely, knockdown of FBXW7 reversed the effects observedupon miR-223-3p inhibition. Our data demonstrate that MiR-223-3ppositively regulates sepsis-induced inflammatory responses by blockingFBXW7 expression, and thus it may provide a new diagnostic markerfor sepsis patients.</p>

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MiR-223-3p Positively Regulates Sepsis-Induced Inflammatory Responses by Targeting the Ubiquitin Ligase FBXW7

  • 期刊论文

  • 发表日期：

    2025年3月1日

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  • Zhaokun Fan,
  • Mingxia Xu,
  • Yingying Shen

摘要

Abstract

A growing amount of research is showing that miRNA plays acritical role in the pathophysiology of sepsis. Nevertheless, itis still unclear how miR-223-3p regulates sepsis. The purpose ofthis study is to look into the relationship and underlying mechanismbetween sepsis and miR-223-3p. We used qRT-PCR to assess RNA expression.Cell apoptosis was detected employing flow cytometry. The relationship betweenmiR-223-3p and its targeted protein was investigated utilizing luciferasereporter assays. Protein expression was analyzed through westernblot and immunofluorescence, while cell viability was determinedusing the CCK-8 assay. The results showed that miR-223-3p was significantlyup-regulated in septic patient serum and LPS-induced RAW264.7 cells,while the expression of FBXW7 showed the opposite pattern. Knockdownof miR-223-3p resulted in a decrease in Bax and cleaved caspase-3 expression,as well as an increase in Bcl-2 expression in LPS-induced RAW264.7cells. Conversely, knockdown of FBXW7 reversed the effects observedupon miR-223-3p inhibition. Our data demonstrate that MiR-223-3ppositively regulates sepsis-induced inflammatory responses by blockingFBXW7 expression, and thus it may provide a new diagnostic markerfor sepsis patients.