Morphology of Doxorubicin-Induced Organopathies under Different Intravenous Nicotinamide Riboside Administration Modes

Doxorubicin (DOX) is a potent chemotherapeutic drug, but itsclinical use is hindered by significant side effects, it exertson such vital organs as the heart, kidneys, lungs, liver, and intestines.Currently, there is a lack of effective drugs that can provide simultaneousprotection of the heart and other vital organs during chemotherapy.Nicotinamide riboside (NR) holds promise as a pharmacological agent, ableto ensure comprehensive protection against DOX systemic toxicity.This study was aimed to evaluate morphological characteristics ofthe vital organs (heart, lungs, liver, kidneys) in male SPF Wistarrats (n = 60, 283 ± 22 g) withchronic doxorubicin-induced cardiomyopathy under various intravenousNR administration modes (1800 mg/kg) as a protective agent. Therats were divided into four groups: (1) intact, (2) control (DOX),(3) combined administration mode (NR+DOX), (4) preventive administrationmode (NR/NR+DOX). Post-administration follow-up period spanned two monthsand ended with tissue sampling with subsequent morphological analysis.The development of DOX-induced cardiotoxicity was monitored by echocardiography.DOX elicited more severe morphological alterations in the heart,kidneys, and lungs compared to the liver. Both NR administrationmodes demonstrated protective effects, with the preventive modeshowing the greatest efficacy in safeguarding the vital organs.