Effect of Genetically Engineered Drugs on Aseptic Femoral Head Necrosis in Rats

Bone tissue is a dynamic structure with a metabolic function.Bone homeostasis is maintained due to its continuous renewal, remodeling.Meanwhile, a number of pathological processes, e.g., ischemia, can leadto homeostatic imbalance in the bone structure. One of these pathologiesis aseptic femoral head necrosis (AFHN). Here, we analyze the expressiondynamics of genes involved in maintaining bone tissue homeostasisand changing the histological picture throughout AFHN developmentin Wistar rats, both untreated with genetically engineered drugsand against the background of IL-6 and TNF-α blockade. After asepticnecrosis induction in the proximal femoral epiphysis, the histologicalpicture was distinct in animals of different experimental groups.Rats treated with genetically engineered drugs exhibited less perturbedbone architectonics and a larger volume of bone lamellae comparedto untreated animals. The latter rat group demonstrated the mostvivid histological picture of osteodestruction and increased geneexpression of pro-inflammatory cytokines. In animals administeredwith IL-6 and TNF-α blockers, cancellous bone mRNA profile in theproximal femoral epiphysis showed an upward tendency of osteoregenerationgene expression. At the same time, rats injected with a monoclonalbody to the IL-6 receptor yielded a maximum inhibition of osteoclastogenicgene expression.