Scholarlink

Abstract <p>An approach has been proposed for assessing the effect of exposure to a low-energy electron beam on structural characteristics of a number of unsaturated fatty acids present in fish oil—oleic, linoleic, arachidonic, eicosapentaenoic, and docosahexaenoic acids—with the use of high-resolution liquid chromatography–mass spectrometry. The subject of this study was commercially available fish oil containing omega-3 and omega-6 polyunsaturated fatty acids. Samples were irradiated with 1-MeV electrons at the UELR-1-25-T-001 accelerator to doses of 0.25, 0.5, 1, 5, and 8 kGy at a dose rate of 10 Gy/s and average beam current of 0.5 μA. The structural integrity of the unsaturated fatty acids chosen was analyzed by high-performance liquid chromatography–tandem mass spectrometry (HPLC–MS/MS). The percentage of intact molecules of the unsaturated fatty acids was evaluated by comparing the peak areas of the analytes in an irradiated solution and unirradiated (control) sample. Taking into account optimization of detection conditions, we calculated the detection limit for each acid and distinguished two possibilities of identifying the presence of unsaturated fatty acids in a sample: reliably detected or not detected. Using the proposed approach, irradiation with low-energy accelerated electrons to doses between 0.25 and 8 kGy at a dose rate of 10 Gy/s has been shown to reduce the concentration of two omega-3 fatty acids—eicosapentaenoic and docosahexaenoic—in aqueous samples.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Influence of Low-Energy Accelerated Electrons on Structural Characteristics of Unsaturated Fatty Acids Studied by High-Resolution Liquid Chromatography–Mass Spectrometry

  • 期刊论文

  • 发表日期：

    2025年1月1日

查看全文
  • A. V. Brown,
  • V. S. Ipatova,
  • U. A. Bliznyuk,
  • P. Yu. Borshchegovskaya,
  • A. P. Chernyaev,
  • I. A. Ananyeva,
  • I. A. Rodin

摘要

Abstract

An approach has been proposed for assessing the effect of exposure to a low-energy electron beam on structural characteristics of a number of unsaturated fatty acids present in fish oil—oleic, linoleic, arachidonic, eicosapentaenoic, and docosahexaenoic acids—with the use of high-resolution liquid chromatography–mass spectrometry. The subject of this study was commercially available fish oil containing omega-3 and omega-6 polyunsaturated fatty acids. Samples were irradiated with 1-MeV electrons at the UELR-1-25-T-001 accelerator to doses of 0.25, 0.5, 1, 5, and 8 kGy at a dose rate of 10 Gy/s and average beam current of 0.5 μA. The structural integrity of the unsaturated fatty acids chosen was analyzed by high-performance liquid chromatography–tandem mass spectrometry (HPLC–MS/MS). The percentage of intact molecules of the unsaturated fatty acids was evaluated by comparing the peak areas of the analytes in an irradiated solution and unirradiated (control) sample. Taking into account optimization of detection conditions, we calculated the detection limit for each acid and distinguished two possibilities of identifying the presence of unsaturated fatty acids in a sample: reliably detected or not detected. Using the proposed approach, irradiation with low-energy accelerated electrons to doses between 0.25 and 8 kGy at a dose rate of 10 Gy/s has been shown to reduce the concentration of two omega-3 fatty acids—eicosapentaenoic and docosahexaenoic—in aqueous samples.