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<b>Abstract</b> <p>Heat shock protein Hsp90 is an important cellular chaperone that contributes to the folding, stabilization, and regulation of specific target proteins involved in many cellular processes. Mammalian cells contain two isoforms of Hsp90, the inducible isoform Hsp90α and the constitutive isoform Hsp90β. Recently, we showed that knockout of the HP90AB1 gene encoding Hsp90β in human HT1080 fibrosarcoma cells (Hsp90β-KO cells) did not affect cell proliferation as well as resistance to heat shock and oxidative stress, while slightly reduced cell migration. This indicates the adaptation of cells and the cellular chaperone machinery to the loss of one of the key cellular chaperones, Hsp90β. In this work, using real-time quantitative PCR and Western blotting, we found that knockout of the gene encoding Hsp90β was accompanied by changes in the expression and content of individual chaperones and co-chaperones associated with Hsp90 and Hsp70 chaperone machineries. In the HSP90 family of chaperones/co-chaperones, an increase in the expression and content of the cytosolic isoform of Hsp90α remaining in the cell and upregulation of a number of important Hsp90 co-chaperones (FKBP4 and HOP) were observed. The expression and content of key chaperones, inducible Hsp70, constitutive Hsc70, and mitochondrial GRP75, were significantly increased in the HSP70 family of chaperones/co-chaperones. As a result, some mechanisms of adaptation of Hsp90 and Hsp70 chaperone machineries in response to the loss of one of the key chaperones, Hsp90β, by cells have been established for the first time. The Hsp90β-KO cells we created that do not express Hsp90β can be considered as a cellular model of pharmacological inhibition of Hsp90β function by promising Hsp90β-specific inhibitors created for tumor therapy. The revealed changes in Hsp90- and Hsp70-associated chaperones and co-chaperones in response to the loss of Hsp90β demonstrate probable changes in the cellular chaperone machinery in response to the suppression of Hsp90β by such inhibitors and can be taken into account when developing combined therapeutic strategies using Hsp90β-specific inhibitors.</p>

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Expression of Cellular Chaperones and Co-Chaperones Associated with Heat Shock Proteins Hsp90 and Hsp70 in Human Fibrosarcoma HT1080 Cells That Do Not Synthesize Hsp90β

  • 期刊论文

  • 发表日期：

    2025年8月1日

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  • V. S. Petrenko,
  • V. V. Vrublevskaya,
  • O. S. Morenkov,
  • Y. Y. Skarga,
  • M. A. Zhmurina

摘要

Abstract

Heat shock protein Hsp90 is an important cellular chaperone that contributes to the folding, stabilization, and regulation of specific target proteins involved in many cellular processes. Mammalian cells contain two isoforms of Hsp90, the inducible isoform Hsp90α and the constitutive isoform Hsp90β. Recently, we showed that knockout of the HP90AB1 gene encoding Hsp90β in human HT1080 fibrosarcoma cells (Hsp90β-KO cells) did not affect cell proliferation as well as resistance to heat shock and oxidative stress, while slightly reduced cell migration. This indicates the adaptation of cells and the cellular chaperone machinery to the loss of one of the key cellular chaperones, Hsp90β. In this work, using real-time quantitative PCR and Western blotting, we found that knockout of the gene encoding Hsp90β was accompanied by changes in the expression and content of individual chaperones and co-chaperones associated with Hsp90 and Hsp70 chaperone machineries. In the HSP90 family of chaperones/co-chaperones, an increase in the expression and content of the cytosolic isoform of Hsp90α remaining in the cell and upregulation of a number of important Hsp90 co-chaperones (FKBP4 and HOP) were observed. The expression and content of key chaperones, inducible Hsp70, constitutive Hsc70, and mitochondrial GRP75, were significantly increased in the HSP70 family of chaperones/co-chaperones. As a result, some mechanisms of adaptation of Hsp90 and Hsp70 chaperone machineries in response to the loss of one of the key chaperones, Hsp90β, by cells have been established for the first time. The Hsp90β-KO cells we created that do not express Hsp90β can be considered as a cellular model of pharmacological inhibition of Hsp90β function by promising Hsp90β-specific inhibitors created for tumor therapy. The revealed changes in Hsp90- and Hsp70-associated chaperones and co-chaperones in response to the loss of Hsp90β demonstrate probable changes in the cellular chaperone machinery in response to the suppression of Hsp90β by such inhibitors and can be taken into account when developing combined therapeutic strategies using Hsp90β-specific inhibitors.