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<b>Abstract</b> <p>Cholesterol plays an important role in the structural organization and functioning of spermatozoa membranes. The purpose of this work is to study the structural changes in human spermatozoa membranes using spin probes after removing cholesterol from them using beta-methylcyclodextrin, which binds cholesterol. Using a model system (liposomes with different cholesterol content), it was shown that the presence of cholesterol in liposomes affected the EPR spectra of the spin probe 5-doxyl stearate dissolved in the lipid bilayer, causing an increase in the microviscosity of the membrane. A study performed on human spermatozoa showed that the extraction of cholesterol from them using beta-methylcyclodextrin was accompanied by an increase in the mobility of the spin probe 5-doxyl stearate, indicating that the removal of cholesterol helps to reduce the microviscosity of the lipid bilayer of spermatozoa membranes.</p>

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The Effect of Cholesterol on Spermatozoa Membranes: Investigation Using Spin Probes

  • 期刊论文

  • 发表日期：

    2025年8月1日

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  • A. G. Mironova,
  • B. V. Trubitsin,
  • E. Yu. Simonenko,
  • A. V. Sybachin,
  • S. A. Yakovenko,
  • A. N. Tikhonov

摘要

Abstract

Cholesterol plays an important role in the structural organization and functioning of spermatozoa membranes. The purpose of this work is to study the structural changes in human spermatozoa membranes using spin probes after removing cholesterol from them using beta-methylcyclodextrin, which binds cholesterol. Using a model system (liposomes with different cholesterol content), it was shown that the presence of cholesterol in liposomes affected the EPR spectra of the spin probe 5-doxyl stearate dissolved in the lipid bilayer, causing an increase in the microviscosity of the membrane. A study performed on human spermatozoa showed that the extraction of cholesterol from them using beta-methylcyclodextrin was accompanied by an increase in the mobility of the spin probe 5-doxyl stearate, indicating that the removal of cholesterol helps to reduce the microviscosity of the lipid bilayer of spermatozoa membranes.