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<b>Abstract</b> <p>The development of cryopreservation methods for testicular spermatozoa makes it possible to safely and economically reproduce genetically diverse agricultural and numerous endangered animal species. The effect of the TSKY peptide (Thr–Ser–Lys–Tyr) isolated from the brains of winter-sleeping ground squirrels (Spermophillus undulatus) in a state of deep hibernation on the physiological activity of bovine sperm cells was studied. A decrease in the activity of bovine rapidly moving spermatozoa was shown when the TSKY peptide was added at the thawing stage after cryopreservation of reproductive cells in liquid nitrogen (–196°C).</p>

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The Effect of the TSKY Peptide on the Physiological Activity of Bovine Spermatozoa

  • 期刊论文

  • 发表日期：

    2025年8月1日

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  • N. A. Ivlicheva,
  • N. V. Shishova,
  • E. N. Gakhova,
  • V. K. Uteshev,
  • R. H. Ziganshin,
  • L. I. Kramarova

摘要

Abstract

The development of cryopreservation methods for testicular spermatozoa makes it possible to safely and economically reproduce genetically diverse agricultural and numerous endangered animal species. The effect of the TSKY peptide (Thr–Ser–Lys–Tyr) isolated from the brains of winter-sleeping ground squirrels (Spermophillus undulatus) in a state of deep hibernation on the physiological activity of bovine sperm cells was studied. A decrease in the activity of bovine rapidly moving spermatozoa was shown when the TSKY peptide was added at the thawing stage after cryopreservation of reproductive cells in liquid nitrogen (–196°C).