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<p>The possibilities of quantitative analysis of weak signals from cells of biochip arrays fixed on a substrate, the fluorescence of which is comparable in intensity to the fluorescence of the substrate, were investigated using a specially designed wide-field digital microscope, a highly sensitive biochip analyzer. In the spectral range of cyanine dye Cy5 (λ<sub>ex</sub> = 645 nm, λ<sub>em</sub> = 665 nm), the relative levels of intrinsic fluorescence of the surfaces of a number of materials that can serve as substrates for biochip matrices used in medical diagnostics were evaluated. It was shown that the analyzer on a substrate of black polybutylene terephthalate (black crastin) allows detecting the fluorescence of 3 and more Sy5 dye molecules/µm<sup>2</sup> on its surface.</p>

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Biochip Analyzer as an Equipment Component in “Lab-on-Chip” Technology

  • 期刊论文

  • 发表日期：

    2025年2月1日

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  • V. E. Barsky,
  • D. A. Yurasov,
  • G. M. Detkov,
  • S. A. Polyakov,
  • R. A. Miftakhov,
  • G. F. Shtylev,
  • I. Yu. Shishkin,
  • V. E. Kuznetsova,
  • V. E. Shershov,
  • V. A. Vasiliskov,
  • O. A. Zasedateleva,
  • A. V. Chudinov

摘要

The possibilities of quantitative analysis of weak signals from cells of biochip arrays fixed on a substrate, the fluorescence of which is comparable in intensity to the fluorescence of the substrate, were investigated using a specially designed wide-field digital microscope, a highly sensitive biochip analyzer. In the spectral range of cyanine dye Cy5 (λex = 645 nm, λem = 665 nm), the relative levels of intrinsic fluorescence of the surfaces of a number of materials that can serve as substrates for biochip matrices used in medical diagnostics were evaluated. It was shown that the analyzer on a substrate of black polybutylene terephthalate (black crastin) allows detecting the fluorescence of 3 and more Sy5 dye molecules/µm2 on its surface.