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Abstract <p>Genome editing using the CRISPR/Cas9 system has become a staple of modern genome manipulation. In&#xa0;its&#xa0;original form, editing involved introducing double-strand breaks into DNA, which can cause genomic instability. The&#xa0;appearance of the first base editors in 2016 expanded the range of editing technologies and enabled single-nucleotide changes to be introduced into the genome through deamination of nucleobases, bypassing the double-strand break stage. Further development of base editors involves the incorporation of additional modules, DNA&#xa0;glycosylases, that can remove modified or even normal nucleobases and create non-instructive apurinic/apyrimidinic sites in&#xa0;DNA, significantly expanding the range of available single-nucleotide substitutions. This review examines the operating principles of the glycosylase base editors, the main limitations of these genome manipulation tools, and promising areas for the development of this technology.</p>

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Glycosylase Base Editors: New Tools for Genome Editing

  • 期刊论文

  • 发表日期：

    2026年7月31日

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  • Liliya M. Kulishova,
  • Dmitry O. Zharkov

摘要

Abstract

Genome editing using the CRISPR/Cas9 system has become a staple of modern genome manipulation. In its original form, editing involved introducing double-strand breaks into DNA, which can cause genomic instability. The appearance of the first base editors in 2016 expanded the range of editing technologies and enabled single-nucleotide changes to be introduced into the genome through deamination of nucleobases, bypassing the double-strand break stage. Further development of base editors involves the incorporation of additional modules, DNA glycosylases, that can remove modified or even normal nucleobases and create non-instructive apurinic/apyrimidinic sites in DNA, significantly expanding the range of available single-nucleotide substitutions. This review examines the operating principles of the glycosylase base editors, the main limitations of these genome manipulation tools, and promising areas for the development of this technology.