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Abstract <p><i>Pulsatilla vulgaris</i> (pasque flower) is a rare and endangered plant species. Using ITS1-5.8S rDNA-ITS2 barcode sequencing and locus-specific NGS of the ITS1 spacer, we studied intraspecific and interspecific polymorphism of 35S rDNA loci and investigated the origin of tetraploid <i>P.&#xa0;vulgaris</i> from a unique Russian population. For the first time, the chromosome number in the karyotype of plants from this population was determined as 2n&#xa0;=&#xa0;32. It was shown that one of the parental species of this tetraploid is the diploid <i>Pulsatilla patens</i> (2n&#xa0;=&#xa0;16), while the identity of the second parental species remains unknown, although it appears to be closely related to the diploid <i>Pulsatilla pratensis</i>.</p>

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Intragenomic Polymorphism of 35S rDNA Internal Transcribed Spacers: Verification of the Hypothesis on the Hybridogenic Origin of Tetraploid Pulsatilla vulgaris

  • 期刊论文

  • 发表日期：

    2026年1月1日

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  • Elizaveta O. Punina,
  • Alexander A. Gnutikov,
  • Nikolay N. Nosov,
  • Victoria S. Shneyer,
  • Alexander V. Rodionov

摘要

Abstract

Pulsatilla vulgaris (pasque flower) is a rare and endangered plant species. Using ITS1-5.8S rDNA-ITS2 barcode sequencing and locus-specific NGS of the ITS1 spacer, we studied intraspecific and interspecific polymorphism of 35S rDNA loci and investigated the origin of tetraploid P. vulgaris from a unique Russian population. For the first time, the chromosome number in the karyotype of plants from this population was determined as 2n = 32. It was shown that one of the parental species of this tetraploid is the diploid Pulsatilla patens (2n = 16), while the identity of the second parental species remains unknown, although it appears to be closely related to the diploid Pulsatilla pratensis.