Scholarlink

<b>Abstract</b>— <p>Optimization of the protocols for cryopreservation and subsequent thawing of primary germ cells in Pushkin breed chicken was carried out. It was detected that with a controlled cooling rate of 1°C/min and combination of penetrating and non-penetrating cryoprotectants, a maximal viability of the cells was 62.9% after their slow thawing at 37°C, which is significantly higher than the values observed during thawing at 4°C. The results obtained emphasize a critical role of thawing temperature in the cell survival, demonstrating that rapid thawing increases their viability by minimizing sublethal damages. The study suggests a more efficient approach to the preservation of poultry genetic resources and creates a potential for biotechnology in the field of animal husbandry.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Peculiarities of Cryopreservation and Decryopreservation of Primordial Germ Cells in the Pushkin Breed of Chickens

  • 期刊论文

  • 发表日期：

    2025年12月1日

查看全文
  • G. K. Peglivanyan,
  • T. A. Larkina,
  • N. R. Reinbach,
  • E. A. Polteva,
  • A. P. Dysin,
  • A. E. Ryabova,
  • A. I. Azovtseva,
  • A. A. Krutikova

摘要

Abstract—

Optimization of the protocols for cryopreservation and subsequent thawing of primary germ cells in Pushkin breed chicken was carried out. It was detected that with a controlled cooling rate of 1°C/min and combination of penetrating and non-penetrating cryoprotectants, a maximal viability of the cells was 62.9% after their slow thawing at 37°C, which is significantly higher than the values observed during thawing at 4°C. The results obtained emphasize a critical role of thawing temperature in the cell survival, demonstrating that rapid thawing increases their viability by minimizing sublethal damages. The study suggests a more efficient approach to the preservation of poultry genetic resources and creates a potential for biotechnology in the field of animal husbandry.