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Abstract <p>The traditional quantitative real-time polymerase chain reaction is the gold standard among molecular diagnostic methods and is used both for the identification of pathogens of various diseases, and for genotyping and standardization of the NGS library. The digital polymerase chain reaction technology is an upgraded version of the polymerase chain reaction, and over the past five years has proven to be more sensitive, specific, and accurate. This article provides an overview of the modern digital polymerase chain reaction platform and their comparative analysis. Special features, uses, and possible applications are selected.</p>

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A State-of-the-Art Digital PCR Platform

  • 期刊论文

  • 发表日期：

    2025年12月9日

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  • Yu. L. Lebedeva,
  • E. A. Cherkashin,
  • V. G. Akimkin

摘要

Abstract

The traditional quantitative real-time polymerase chain reaction is the gold standard among molecular diagnostic methods and is used both for the identification of pathogens of various diseases, and for genotyping and standardization of the NGS library. The digital polymerase chain reaction technology is an upgraded version of the polymerase chain reaction, and over the past five years has proven to be more sensitive, specific, and accurate. This article provides an overview of the modern digital polymerase chain reaction platform and their comparative analysis. Special features, uses, and possible applications are selected.