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<b>Abstract</b>— <p>The isolation and identification to species of 85 strains of osmotolerant yeasts related to Ascomycota from bees and bee-associated substrates, such as honeycombs, bee bread, and honey flowers collected in the republics of the Caucasus region including Dagestan, Kabardino-Balkaria, North Ossetia, and the Chechen Republic, as well as from candied fruits and fruit leather, are reported. Due to elective selection conditions and specific substrates, the isolated yeasts did not show much species diversity. Representatives of the genera <i>Zygosaccharomyces</i> and <i>Starmerella</i> prevailed among the isolated osmotolerant yeasts. When the levels of glycerol accumulation by the representatives of the identified genera were studied, the highest glycerol production in the absence of sulfites inhibiting the fermentation process was recorded in the Y-5206 and Y-5190 strains belonging to the species <i>Pichia kudriavzevii</i>, namely, 5.9 and 7.8 g/L, respectively. Under the sulfite stress conditions, when the medium pH was 8.0, the highest glycerol production level of 23.5 g/L was recorded in the <i>S. cerevisiae</i> Y-5189 yeast. The highest rate of glucose-to-glycerol conversion (46.3%) was shown by the <i>Zygosaccharomyces roxii</i> yeast strain Y-5182 in the presence of sulfites. The strains of osmotolerant yeasts belonging to the species <i>Zygosaccharomyces roxii</i>, as well as to all species in the genus <i>Starmerella</i>, which are characterized by the highest glucose-to-glycerol conversion levels at a low level of ethanol production and are able to accumulate extracellular glycerol efficiently in the absence of sulfite stress, may be of potential interest for further studies aimed at developing biotechnologies for production of this alcohol.</p>

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Osmotolerant Yeasts Associated with Bees and Sugar-Enriched Substrates: Taxonomy and Ability to Synthesize Glycerol

  • 期刊论文

  • 发表日期：

    2025年12月9日

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  • M. M. Vustin,
  • M. A. Velikaya,
  • G. S. Kachmazov,
  • A. Yu. Tuaeva,
  • A. N. Revazova,
  • S. P. Sineokiy

摘要

Abstract—

The isolation and identification to species of 85 strains of osmotolerant yeasts related to Ascomycota from bees and bee-associated substrates, such as honeycombs, bee bread, and honey flowers collected in the republics of the Caucasus region including Dagestan, Kabardino-Balkaria, North Ossetia, and the Chechen Republic, as well as from candied fruits and fruit leather, are reported. Due to elective selection conditions and specific substrates, the isolated yeasts did not show much species diversity. Representatives of the genera Zygosaccharomyces and Starmerella prevailed among the isolated osmotolerant yeasts. When the levels of glycerol accumulation by the representatives of the identified genera were studied, the highest glycerol production in the absence of sulfites inhibiting the fermentation process was recorded in the Y-5206 and Y-5190 strains belonging to the species Pichia kudriavzevii, namely, 5.9 and 7.8 g/L, respectively. Under the sulfite stress conditions, when the medium pH was 8.0, the highest glycerol production level of 23.5 g/L was recorded in the S. cerevisiae Y-5189 yeast. The highest rate of glucose-to-glycerol conversion (46.3%) was shown by the Zygosaccharomyces roxii yeast strain Y-5182 in the presence of sulfites. The strains of osmotolerant yeasts belonging to the species Zygosaccharomyces roxii, as well as to all species in the genus Starmerella, which are characterized by the highest glucose-to-glycerol conversion levels at a low level of ethanol production and are able to accumulate extracellular glycerol efficiently in the absence of sulfite stress, may be of potential interest for further studies aimed at developing biotechnologies for production of this alcohol.