Scholarlink

Abstract <p>The strain INA 01478 has been isolated from the soil of bottom sediments of Trondheim Fjord, Norwegian Sea. This halotolerant strain is capable of producing the isoflavone daidzein in the culture medium. The preliminary genome analysis has identified homologs of genes involved in the de novo biosynthesis of daidzein in the genome of the strain. Structural elucidation, performed using 2D NMR and HR-ESIMS data, has identified the compound as daidzein. The absolute configurations of these stereoisomers have been determined through conformational NMR analysis and circular dichroism spectroscopy. The antifungal activity of daidzein has been detected against <i>Candida albicans</i> ATCC 2091, <i>Saccharomyces cerevisiae</i> INA 01042, opportunistic filamentous fungi <i>Aspergillus niger</i> ATCC 16404, and <i>Fusarium oxysporum</i> VKPM&#xa0;F-148.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Halotolerant Streptomyces albidoflavus INA 01478 as a Producer of Daidzein: Genome Annotation, purification and Antifungal Activity

  • 期刊论文

  • 发表日期：

    2025年8月17日

查看全文
  • O. N. Sineva,
  • N. N. Markelova,
  • K. V. Malysheva,
  • O. A. Kolpakova,
  • G. Kh. Kudryakova,
  • I. A. Prokhorenko,
  • O. V. Kisil,
  • A. Yu. Simonov,
  • V. I. Polshakov,
  • I. B. Levshin,
  • V. S. Sadykova

摘要

Abstract

The strain INA 01478 has been isolated from the soil of bottom sediments of Trondheim Fjord, Norwegian Sea. This halotolerant strain is capable of producing the isoflavone daidzein in the culture medium. The preliminary genome analysis has identified homologs of genes involved in the de novo biosynthesis of daidzein in the genome of the strain. Structural elucidation, performed using 2D NMR and HR-ESIMS data, has identified the compound as daidzein. The absolute configurations of these stereoisomers have been determined through conformational NMR analysis and circular dichroism spectroscopy. The antifungal activity of daidzein has been detected against Candida albicans ATCC 2091, Saccharomyces cerevisiae INA 01042, opportunistic filamentous fungi Aspergillus niger ATCC 16404, and Fusarium oxysporum VKPM F-148.