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Abstract <p>The potential of using phage display technology to obtain antigentamicin antibodies has been demonstrated. For the first time antigentamicin recombinant antibodies were obtained using a sheep phage library (Griffin.1, United Kingdom). The interaction between the obtained phage antibodies with gentamicin was monitored using a circular dichroism spectrometer. It was shown that the interaction of antigentamicin phage antibodies with the corresponding antibiotic is characterized by the presence of a characteristic exciton doublet: a positive peak at ~233 nm and a more intense negative peak with a maximum at ~240 nm. The possibility of gentamicin indication using a test system based on the dot immunoassay method and antigentamicin recombinant antibodies in aqueous solutions has been demonstrated for the first time; the lower limit of antibiotic detection is 0.5 μg/mL. Using the dot immunoassay method, it was found that antigentamicin phage antibodies did not exhibit activity towards ampicillin and tetracycline but showed activity towards kanamycin (lower limit of detection, 1 μg/mL). The results are promising for further development of methods for gentamicin detection using recombinant phage antibodies.</p>

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Production of Recombinant Phage Antibodies Specific to Gentamicin and Their Use in Dot Immunoassay

  • 期刊论文

  • 发表日期：

    2025年8月6日

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  • O. I. Guliy,
  • D. S. Chumakov,
  • V. S. Grinev,
  • O. A. Karavaeva

摘要

Abstract

The potential of using phage display technology to obtain antigentamicin antibodies has been demonstrated. For the first time antigentamicin recombinant antibodies were obtained using a sheep phage library (Griffin.1, United Kingdom). The interaction between the obtained phage antibodies with gentamicin was monitored using a circular dichroism spectrometer. It was shown that the interaction of antigentamicin phage antibodies with the corresponding antibiotic is characterized by the presence of a characteristic exciton doublet: a positive peak at ~233 nm and a more intense negative peak with a maximum at ~240 nm. The possibility of gentamicin indication using a test system based on the dot immunoassay method and antigentamicin recombinant antibodies in aqueous solutions has been demonstrated for the first time; the lower limit of antibiotic detection is 0.5 μg/mL. Using the dot immunoassay method, it was found that antigentamicin phage antibodies did not exhibit activity towards ampicillin and tetracycline but showed activity towards kanamycin (lower limit of detection, 1 μg/mL). The results are promising for further development of methods for gentamicin detection using recombinant phage antibodies.