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<b>Abstract</b>— <p>This study presents a method for the co-amplification of α- and β-hemolysin genes in bacteria of the genus <i>Aeromonas</i>. The developed multiplex PCR protocol demonstrated high specificity and sensitivity. PCR screening showed a wide distribution of hemolysin genes among <i>Aeromonas</i> isolates from aquaculture and environmental sites. A correlation was found between the hemolytic activity of strains and the presence of certain genes (<i>hlyA</i> and <i>aerA</i>). The proposed method may become a useful tool for epidemiological monitoring of the spread of pathogenic aeromonad species, which is important for ensuring biological safety of fish production.</p>

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Detection of Hemolytic Strains of Aeromonads by Multiplex PCR

  • 期刊论文

  • 发表日期：

    2025年8月6日

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  • D. A. Dokolin,
  • M. N. Sokolov,
  • A. A. Payuta,
  • E. A. Flerova,
  • Y. V. Zaitseva

摘要

Abstract—

This study presents a method for the co-amplification of α- and β-hemolysin genes in bacteria of the genus Aeromonas. The developed multiplex PCR protocol demonstrated high specificity and sensitivity. PCR screening showed a wide distribution of hemolysin genes among Aeromonas isolates from aquaculture and environmental sites. A correlation was found between the hemolytic activity of strains and the presence of certain genes (hlyA and aerA). The proposed method may become a useful tool for epidemiological monitoring of the spread of pathogenic aeromonad species, which is important for ensuring biological safety of fish production.