Proteome Analysis of the Nitric Oxide Donor Effect on Pisum Sativum L. Roots

Proteomic analysis of the NO donor–sodium nitroprusside effect on pea seedlings roots revealed a high degree of induction of the platform propeller protein WD40, PR10, ferritin, and the chitinase-like protein. Less induction was observed for ABR17, the endochitinase-like protein, enzymes of phytoalexins synthesis, and proline iminopeptidase, which releases proline from proline-enriched proteins, and UTP-glucose-1-P-uridylyl transferase involved in the synthesis of cellulose and callose. Some of these defense compounds, such as endochitinase, chitinase-like protein, and phenolic phytoalexins, are transported into the rhizosphere and may influence the rhizosphere microbiome. There are examples of the use of NO defense properties in agrotechnology both for treating plants with NO donors and for creating stress-resistant transgenic plants with a high content of NO or compounds induced by it (ferritin, etc.).