Disruption of RGD1 Gene Improves Acetic Acid Tolerance in Saccharomyces cerevisiae

This study aimed to identify genes related to acetic acid tolerance and elucidate the tolerance mechanism in yeast Saccharomyces cerevisiae as an appropriate eukaryote model. In this study, one tolerant strain exposed to acetic acid stress was isolated by screening a transposon-mediated mutant library and the disrupted gene was identified to be RGD1. In addition, this phenotype related to acetic acid tolerance was confirmed by deletion and overexpressing of this corresponding gene. This mutant strain showed activation of Hog1p and increased expression of the MSN2/4 and STRE-mediated genes such as CTT1 and HSP12 in response to acetic acid stress.