<p>Declining birthrates, an increasing number of older adults, and rising recognition of individuals with disabilities and various sexual orientations necessitate fostering societies where diverse individuals maintain high well-being irrespective of gender, age, parenting roles, disability, and minority or majority status. Although some scholars have developed or used scales to measure tolerance and autonomy, these existing scales are not necessarily developed with an orientation toward inclusive societies. To address this need, we developed and validated new scales of tolerance and autonomy, guided by a perspective of inclusive communities and societies where individuals are surrounded by tolerance and act autonomously. We began our study by conducting interviews among diverse groups, including parents, older adults with long-term care needs, and people with disabilities to identify components of tolerant and autonomous behaviors. This qualitative analysis informed the creation of two 20-item self-reported scales for both concepts. These scales were assessed through three online surveys. In Survey 1 (<i>n</i> = 176), we performed an assessment of content validity and exploratory factor analyses; in Survey 2 (<i>n</i> = 1453) and Survey 3 (<i>n</i> = 341), we carried out confirmatory factor analysis, hypothesis testing (association with subjective well-being [SWB]), and assessment of reliabilities (internal consistency, test re-test reliability, and error of measurement). Results revealed that use of both scales was valid, reliable, and two scale scores were positively associated with the SWB score. Additionally, we updated the definitions of tolerance and autonomy through qualitative and quantitative research. The tolerance and autonomy scales have potentials to be applicable in many diverse settings and serve as practical tools for deepening the understanding of the diverse challenges individuals, communities, and organizations face, leading to better solutions.</p>