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<p>Intracellular phagosomes have a lipid bilayer-encapsulated fluidic shell outside the particle, on the outer side of which, molecular motors are attached. An optically trapped spherical birefringent particle phagosome provides an ideal platform to probe fluidity of the shell, as the inner particle is optically confined both in translation and in rotation. Using a recently reported method to calibrate the translation and pitch rotations - yielding a spatial resolution of about 2 nm and angular resolution of 0.1 degrees - we report novel roto-translational coupled dynamics. We also suggest a new technique where we explore the correlation between the translation and pitch rotation to study extent of activity. Given that a spherical birefringent particle phagosome is almost a sphere, the fact that it turns due to the activity of the motors is not obvious, even implying high rigidity of shell. Applying a minimal model for the roto-translational coupling, we further show that this coupling manifests itself as sustained fluxes in phase space, a signature of broken detailed balance.</p>

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Intracellular phagosome shell is rigid enough to transfer outside torque to the inner spherical particle

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年11月5日

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  • Srestha Roy,
  • Arvin Gopal Subramaniam,
  • Snigdhadev Chakraborty,
  • Jayesh Goswami,
  • Subastri Ariraman,
  • Krishna Kumari Swain,
  • Swathi Sudhakar,
  • Rajesh Singh,
  • Basudev Roy

摘要

Intracellular phagosomes have a lipid bilayer-encapsulated fluidic shell outside the particle, on the outer side of which, molecular motors are attached. An optically trapped spherical birefringent particle phagosome provides an ideal platform to probe fluidity of the shell, as the inner particle is optically confined both in translation and in rotation. Using a recently reported method to calibrate the translation and pitch rotations - yielding a spatial resolution of about 2 nm and angular resolution of 0.1 degrees - we report novel roto-translational coupled dynamics. We also suggest a new technique where we explore the correlation between the translation and pitch rotation to study extent of activity. Given that a spherical birefringent particle phagosome is almost a sphere, the fact that it turns due to the activity of the motors is not obvious, even implying high rigidity of shell. Applying a minimal model for the roto-translational coupling, we further show that this coupling manifests itself as sustained fluxes in phase space, a signature of broken detailed balance.