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<p>Natural enzymes are vital for biosensors but are limited by stability and scalability. Nanozymes, synthetic nanomaterials mimicking enzymes, offer enhanced stability, scalable production, and multifunctional properties. Coupled with aptamers, they enable highly selective biosensing. This review highlights strategies for nanozyme aptasensors using three formats, namely nanozyme and aptamer-based immunosorbent assays (NAISA), adsorption/desorption-based nanozymes, and amplification-based nanozyme approaches, and discusses key considerations during fabrication and future directions for advancing biosensing applications.</p>

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Advancing nanozyme aptasensors status quo via strategies and key fabrication considerations

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年7月8日

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  • Pabudi Weerathunge,
  • Gayatri Bagree,
  • Sanje Mahasivam,
  • Erangi Amarasinghe,
  • Rajesh Ramanathan,
  • Vipul Bansal

摘要

Natural enzymes are vital for biosensors but are limited by stability and scalability. Nanozymes, synthetic nanomaterials mimicking enzymes, offer enhanced stability, scalable production, and multifunctional properties. Coupled with aptamers, they enable highly selective biosensing. This review highlights strategies for nanozyme aptasensors using three formats, namely nanozyme and aptamer-based immunosorbent assays (NAISA), adsorption/desorption-based nanozymes, and amplification-based nanozyme approaches, and discusses key considerations during fabrication and future directions for advancing biosensing applications.