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<p>Emerging zoonotic pathogens represent an escalating threat to global health, underscoring the need for rapid and effective testing strategies. Electrochemical biosensors offer a promising solution for point-of-care testing, enabling sensitive and real-time detection of these pathogens. A major challenge in biosensor technology is biofouling, caused by nonspecific adsorption in complex matrices, which compromises sensor reliability. This study reports a biofouling-resistant electrochemical capacitive biosensor (ECB) capable of rapid (&lt;5 min) detection of zoonotic influenza, specifically avian influenza H5N1 and H1N1 viruses, with detection limits below 50 viral RNA copies/mL. The ECB maintains sensitivity for virus detection and demonstrates excellent signal stability (±3% signal drift) when tested under extreme fouling-prone media. This novel characteristic of the biosensor is achieved by functionalizing its working electrode with a hybrid composite of poly(3,4-ethylenedioxythiophene):polystyrene sulfonate (PEDOT:PSS) and reduced graphene oxide. This capacitive interface supports stable strain-specific aptamer immobilization, providing a robust platform for POCT diagnostics of zoonotic pathogens in real-world settings.</p>

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Biofouling-resistant functionalized capacitive biosensor for rapid detection of zoonotic influenza

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年5月4日

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  • Dishit P. Ghumra,
  • Meng Xu,
  • Ananya Benegal,
  • Michael D. Vahey,
  • Astha Joshi,
  • Kuljeet Seehra,
  • Adrianus C. M. Boon,
  • Rajan K. Chakrabarty

摘要

Emerging zoonotic pathogens represent an escalating threat to global health, underscoring the need for rapid and effective testing strategies. Electrochemical biosensors offer a promising solution for point-of-care testing, enabling sensitive and real-time detection of these pathogens. A major challenge in biosensor technology is biofouling, caused by nonspecific adsorption in complex matrices, which compromises sensor reliability. This study reports a biofouling-resistant electrochemical capacitive biosensor (ECB) capable of rapid (<5 min) detection of zoonotic influenza, specifically avian influenza H5N1 and H1N1 viruses, with detection limits below 50 viral RNA copies/mL. The ECB maintains sensitivity for virus detection and demonstrates excellent signal stability (±3% signal drift) when tested under extreme fouling-prone media. This novel characteristic of the biosensor is achieved by functionalizing its working electrode with a hybrid composite of poly(3,4-ethylenedioxythiophene):polystyrene sulfonate (PEDOT:PSS) and reduced graphene oxide. This capacitive interface supports stable strain-specific aptamer immobilization, providing a robust platform for POCT diagnostics of zoonotic pathogens in real-world settings.