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<p>Coronaviruses (CoVs) pose a persistent global health threat, with animal reservoirs such as bats, rodents, and poultry driving their emergence. Rapid, field-deployable detection tools are urgently needed to monitor these viruses at the animal-human interface, where spillover events are most likely. We present a nanophotonic interferometric biosensor for label-free, amplification-free detection of alpha/beta- and gamma-CoVs in animal reservoirs. The biosensor achieves limits of detection below 10 copies/μL for in vitro transcribed (IVT) RNA samples, strongly correlating with PCR assays. Field validation using RNA extracts from bats and chickens yielded sensitivities of 72–90% and specificities of 83–90%, confirming robust performance across diagnostic relevant ranges. Fabricated on silicon microchips via standard techniques, the biosensor platform is scalable, cost-effective, and compatible with miniaturized components suitable for point-of-care and decentralized use. Our biosensor device addresses a critical gap in veterinary diagnostics, supporting early surveillance and pandemic preparedness under a One Health framework.</p>

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Rapid screening of animal coronaviruses in livestock and wildlife using a silicon photonics biosensor

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年5月4日

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  • Beatriz Serrano,
  • Maria Soler,
  • Céline Courtillon,
  • Chantal Allée,
  • Abir Monastiri,
  • Marc López-Roig,
  • Jordi Serra-Cobo,
  • Laura M. Lechuga

摘要

Coronaviruses (CoVs) pose a persistent global health threat, with animal reservoirs such as bats, rodents, and poultry driving their emergence. Rapid, field-deployable detection tools are urgently needed to monitor these viruses at the animal-human interface, where spillover events are most likely. We present a nanophotonic interferometric biosensor for label-free, amplification-free detection of alpha/beta- and gamma-CoVs in animal reservoirs. The biosensor achieves limits of detection below 10 copies/μL for in vitro transcribed (IVT) RNA samples, strongly correlating with PCR assays. Field validation using RNA extracts from bats and chickens yielded sensitivities of 72–90% and specificities of 83–90%, confirming robust performance across diagnostic relevant ranges. Fabricated on silicon microchips via standard techniques, the biosensor platform is scalable, cost-effective, and compatible with miniaturized components suitable for point-of-care and decentralized use. Our biosensor device addresses a critical gap in veterinary diagnostics, supporting early surveillance and pandemic preparedness under a One Health framework.