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<p>This perspective discusses the future role of all-optical label-free single-molecule sensing. After summarizing the core working principles of the different detection modalities we discuss future research directions in terms of technology development and applications. In terms of future technologies we address research directions in the areas of multi-parametric and interaction free sensors, integration and miniaturization, and advanced data analysis. We further describe potential applications towards probing of protein interactions, DNA and protein sequencing, and molecular diagnostics.</p>

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Future directions in all-optical label-free single-molecule sensing

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年1月13日

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  • Koji Masuda,
  • Reuven Gordon,
  • Philipp Kukura,
  • Christoph Langhammer,
  • Sang-Hyun Oh,
  • Frank Vollmer,
  • Peter Zijlstra

摘要

This perspective discusses the future role of all-optical label-free single-molecule sensing. After summarizing the core working principles of the different detection modalities we discuss future research directions in terms of technology development and applications. In terms of future technologies we address research directions in the areas of multi-parametric and interaction free sensors, integration and miniaturization, and advanced data analysis. We further describe potential applications towards probing of protein interactions, DNA and protein sequencing, and molecular diagnostics.