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<p>Localized surface plasmon resonance (LSPR) is an optical phenomenon derived from the dielectric properties of noble metals, resulting in a highly change-sensitive spectrum that can be used on a sensor platform. We have used gold nanorods to develop a diagnostic assay to detect antibodies against the nucleocapsid (N) protein of SARS-CoV-2. This approach is particularly valuable, considering the limited role of serology in recognizing acute infections. Gold nanoparticles were coated with the recombinant N protein and characterized by spectroscopy, fluorometry, and electron microscopy. Positive and negative COVID-19 sera samples were initially categorized through qRT-PCR and further validated using an anti-IgG ELISA. The nanosensor was accurate and able to detect very low levels of anti-SARS-CoV-2 antibodies derived from different virus variants early in infection (before 10 days post-infection). The nanoplatform exhibited high sensitivity and specificity, is suitable for mass production, and has easy implementation and automatic read-out.</p>

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Serologic LSPR-nanosensor against SARS-COV-2 antibodies and related variants outperforms ELISA in sensitivity

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年3月3日

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  • Alice F. Versiani,
  • Lídia M. Andrade,
  • Thaís F. S. Moraes,
  • Estefânia M. N. Martins,
  • Flávia F. Bagno,
  • Luis A. F. Andrade,
  • Guilherme R. F. Campos,
  • Thayza M. I. L. dos Santos,
  • Marília M. Moraes,
  • Sarah Aparecida Rodrigues Sérgio,
  • Roberto M. Paniago,
  • Felipe M. F. Teixeira,
  • Luiz O. Ladeira,
  • Clascídia A. Furtado,
  • Mauricio L. Nogueira,
  • Jhonattan C. Ramirez,
  • Flávio G. da Fonseca

摘要

Localized surface plasmon resonance (LSPR) is an optical phenomenon derived from the dielectric properties of noble metals, resulting in a highly change-sensitive spectrum that can be used on a sensor platform. We have used gold nanorods to develop a diagnostic assay to detect antibodies against the nucleocapsid (N) protein of SARS-CoV-2. This approach is particularly valuable, considering the limited role of serology in recognizing acute infections. Gold nanoparticles were coated with the recombinant N protein and characterized by spectroscopy, fluorometry, and electron microscopy. Positive and negative COVID-19 sera samples were initially categorized through qRT-PCR and further validated using an anti-IgG ELISA. The nanosensor was accurate and able to detect very low levels of anti-SARS-CoV-2 antibodies derived from different virus variants early in infection (before 10 days post-infection). The nanoplatform exhibited high sensitivity and specificity, is suitable for mass production, and has easy implementation and automatic read-out.