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<p>The classical models of intra-Golgi transport envision a movement of cargoes from <i>cis</i>- to <i>trans</i>-Golgi, followed by their sorting at the <i>trans</i>-Golgi network (TGN). During this vectorial transport, the cargoes are processed by sequentially acting glycosylation enzymes. A number of studies challenged the vectorial transport model and proposed alternative transport routes bypassing either directional transport or the TGN. We have re-visited intra-Golgi transport using varied cargo synchronization protocols, employing both imaging and biochemical methods. We find that cargoes move vectorially across the Golgi stack and reach the TGN. Cell type-dependent variations in transport kinetics and the limited resolution of fluorescence microscopy could have influenced earlier discrepant interpretations. Further, we find that exit from TGN is a rate-limiting step leading to the accumulation of cargoes there before their monoexponential exit. These findings support an intriguing model of intra-Golgi transport, which involves classical vectorial transport across the Golgi stack followed by a non-maturation-based transport from the stack to the TGN.</p>

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Cargoes move from cis to trans-Golgi compartments and concentrate in the TGN before exiting

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年9月3日

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  • Marinella Pirozzi,
  • Ilenia Agliarulo,
  • Rosaria Di Martino,
  • Vincenzo Manuel Marzullo,
  • Micaela Quarto,
  • Gabriele Turacchio,
  • Galina V Beznoussenko,
  • Domenico Russo,
  • Alberto Luini,
  • Alexander A Mironov,
  • Seetharaman Parashuraman

摘要

The classical models of intra-Golgi transport envision a movement of cargoes from cis- to trans-Golgi, followed by their sorting at the trans-Golgi network (TGN). During this vectorial transport, the cargoes are processed by sequentially acting glycosylation enzymes. A number of studies challenged the vectorial transport model and proposed alternative transport routes bypassing either directional transport or the TGN. We have re-visited intra-Golgi transport using varied cargo synchronization protocols, employing both imaging and biochemical methods. We find that cargoes move vectorially across the Golgi stack and reach the TGN. Cell type-dependent variations in transport kinetics and the limited resolution of fluorescence microscopy could have influenced earlier discrepant interpretations. Further, we find that exit from TGN is a rate-limiting step leading to the accumulation of cargoes there before their monoexponential exit. These findings support an intriguing model of intra-Golgi transport, which involves classical vectorial transport across the Golgi stack followed by a non-maturation-based transport from the stack to the TGN.