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<p>Glioblastoma multiforme (GBM) is the most lethal form of malignant brain tumor in adults. Dysregulation of protein synthesis contributes to cancer cell plasticity, driving GBM cell heterogeneity, metastatic behavior, and drug resistance. Understanding the complex network and signaling pathways governing protein translation, is therefore an important goal for GBM treatment. Here we identify a novel signaling network centered on the E3 ubiquitin ligase praja2 that controls protein translation in GBM. Praja2 forms a multimeric complex with the RNA helicase DDX6, which inhibits translation of target RNAs within processing bodies (P-bodies). Stimulation of cAMP signaling through activation of G-protein-coupled receptors induces P-body assembly through praja2-mediated non-proteolytic polyubiquitylation of DDX6. Genetic inactivation of praja2 reshapes DDX6/mRNA complexes and translating polysomes and promotes cellular senescence and GBM growth arrest. Expression of an ubiquitylation-defective DDX6 mutant suppresses the assembly of P-bodies and sustains GBM growth. Taken together, our findings identify a cAMP-driven network that controls translation in P-bodies and GBM growth.</p>

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Praja2 controls P-body assembly and translation in glioblastoma by non-proteolytic ubiquitylation of DDX6

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年3月27日

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  • Emanuela Senatore,
  • Rosario Avolio,
  • Laura Rinaldi,
  • Francesco Chiuso,
  • Maria A Oliva,
  • Chiara D’Ambrosio,
  • Antonio Giuseppe Bianco,
  • Emiliano Dalla,
  • Stefano Maria Pagnotta,
  • Raffaella Flammia,
  • Concetta Ambrosino,
  • Domenico Memoli,
  • Gabriele Turacchio,
  • Sonia Ines Mimoune,
  • Yves Toiron,
  • Stephane Audebert,
  • Luc Camoin,
  • Luca Lignitto,
  • Andrea Scaloni,
  • Antonietta Arcella,
  • Antonio Feliciello

摘要

Glioblastoma multiforme (GBM) is the most lethal form of malignant brain tumor in adults. Dysregulation of protein synthesis contributes to cancer cell plasticity, driving GBM cell heterogeneity, metastatic behavior, and drug resistance. Understanding the complex network and signaling pathways governing protein translation, is therefore an important goal for GBM treatment. Here we identify a novel signaling network centered on the E3 ubiquitin ligase praja2 that controls protein translation in GBM. Praja2 forms a multimeric complex with the RNA helicase DDX6, which inhibits translation of target RNAs within processing bodies (P-bodies). Stimulation of cAMP signaling through activation of G-protein-coupled receptors induces P-body assembly through praja2-mediated non-proteolytic polyubiquitylation of DDX6. Genetic inactivation of praja2 reshapes DDX6/mRNA complexes and translating polysomes and promotes cellular senescence and GBM growth arrest. Expression of an ubiquitylation-defective DDX6 mutant suppresses the assembly of P-bodies and sustains GBM growth. Taken together, our findings identify a cAMP-driven network that controls translation in P-bodies and GBM growth.