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<p>Recent developments in single-cell -omic and metabolite imaging technologies and the increasing availability of high-quality genome assemblies are having a transformative impact on the way research is carried out into plant specialised metabolism. Integrating these technologies into pathway discovery projects is therefore highly advantageous. Here, we present a general introduction into methods and workflows in specialised metabolism research. We review a range of recent methodologies, highlighting what they might be used for and common pitfalls which may be encountered. Finally, we provide a practical guide on how these technologies may be incorporated into a specialised metabolic pathway discovery pipeline for researchers who are new to the field.</p>

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A million shades of green: understanding and harnessing plant metabolic diversity

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年7月3日

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  • Rocky D Payet,
  • Adnane Aouidate,
  • Rebecca Casson,
  • Alan Houghton,
  • Mai-Truc Pham,
  • Anne Osbourn

摘要

Recent developments in single-cell -omic and metabolite imaging technologies and the increasing availability of high-quality genome assemblies are having a transformative impact on the way research is carried out into plant specialised metabolism. Integrating these technologies into pathway discovery projects is therefore highly advantageous. Here, we present a general introduction into methods and workflows in specialised metabolism research. We review a range of recent methodologies, highlighting what they might be used for and common pitfalls which may be encountered. Finally, we provide a practical guide on how these technologies may be incorporated into a specialised metabolic pathway discovery pipeline for researchers who are new to the field.