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<p>RNA <i>N</i><sup>6</sup>-methyladenosine (m<sup>6</sup>A, m6A) modification is a critical regulator for a range of physiological processes. However, the dynamic m<sup>6</sup>A profiles within human preimplantation embryos remain uncharacterized. Here, we present the first RNA m<sup>6</sup>A landscape of single human oocytes and early embryos. Comparative analyses with mouse data reveal an intriguing divergence during the window of zygotic genome activation. m<sup>6</sup>A-modified genes are involved in regulation of gene transcription, while unmodified genes are mainly associated with basic metabolic processes. Maternal decay mRNAs exhibit a propensity for m<sup>6</sup>A modifications, and these genes are targeted by miRNAs. m<sup>6</sup>A modified genes that are constantly expressed across all stages demonstrate higher translation efficiency. Moreover, we observe frequent m<sup>6</sup>A enrichment on stage-specifically expressed retrotransposons, particularly within young subfamilies. m<sup>6</sup>A inhibitor leads to m<sup>6</sup>A erasure on massive retrotransposons. In summary, this study provides a resource to broaden our understanding about the regulatory roles of m<sup>6</sup>A during early human embryo development.</p>

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The RNA m6A landscape during human oocyte-to-embryo transition

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年6月4日

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  • Yanjiao Li,
  • Yunhao Wang,
  • Aylin Cengiz,
  • Kang-Xuan Jin,
  • Blanca Corral Castroviejo,
  • Xiaolin Lin,
  • Marie Indahl,
  • Rujuan Zuo,
  • Trine Skuland,
  • Madeleine Fosslie,
  • Maria Biba,
  • Xuechen Wu,
  • Peter Fedorcsak,
  • Magnar Bjørås,
  • Adam Filipczyk,
  • John Arne Dahl,
  • Gareth D Greggains,
  • Kin Fai Au,
  • Arne Klungland

摘要

RNA N6-methyladenosine (m6A, m6A) modification is a critical regulator for a range of physiological processes. However, the dynamic m6A profiles within human preimplantation embryos remain uncharacterized. Here, we present the first RNA m6A landscape of single human oocytes and early embryos. Comparative analyses with mouse data reveal an intriguing divergence during the window of zygotic genome activation. m6A-modified genes are involved in regulation of gene transcription, while unmodified genes are mainly associated with basic metabolic processes. Maternal decay mRNAs exhibit a propensity for m6A modifications, and these genes are targeted by miRNAs. m6A modified genes that are constantly expressed across all stages demonstrate higher translation efficiency. Moreover, we observe frequent m6A enrichment on stage-specifically expressed retrotransposons, particularly within young subfamilies. m6A inhibitor leads to m6A erasure on massive retrotransposons. In summary, this study provides a resource to broaden our understanding about the regulatory roles of m6A during early human embryo development.