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<p>Chloride (Cl<sup>−</sup>) ions cause major damage to crops in saline soils. Understanding the key factors that influence Cl<sup>−</sup> uptake and translocation will aid the breeding of more salt-tolerant crops. Here, using genome-wide association study and transcriptomic analysis, we identified a NITRATE TRANSPORTER 1 (NRT1)/PEPTIDE TRANSPORTER family (NPF) protein, GmNPF7.5, as the dominant gene locus influencing Cl<sup>−</sup> homeostasis in soybean (Glycine max). A natural SNP variation resulted in two haplotypes (GmNPF7.5<sup>HapA</sup> and GmNPF7.5<sup>HapB</sup>), which was associated with Cl<sup>−</sup> content. GmNPF7.5<sup>HapA</sup> mediated Cl<sup>−</sup> or nitrate (NO<sub>3</sub><sup>−</sup>) uptake in a pH-dependent manner and exhibited higher permeability for Cl<sup>−</sup> over NO<sub>3</sub><sup>−</sup>. The suppression of <i>GmNPF7.5</i><sup><i>HapA</i></sup> expression decreased Cl<sup>−</sup> accumulation and salt damage in plants, whereas its overexpression showed the opposite effects. The elite haplotype GmNPF7.5<sup>HapB</sup> diminished Cl<sup>−</sup> transport activity independently from NO<sub>3</sub><sup>−</sup> permeability, thus enhancing soybean salt tolerance. Furthermore, the protein kinase GmPI4Kγ4 could phosphorylate GmNPF7.5, which repressed Cl<sup>−</sup> uptake without affecting NO<sub>3</sub><sup>−</sup> permeability. Our findings define a regulatory mechanism for Cl<sup>−</sup> control under NaCl stress, providing a strategy for the improvement of salt tolerance in soybean plants.</p>

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A phosphorylation-regulated NPF transporter determines salt tolerance by mediating chloride uptake in soybean plants

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年1月3日

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  • Yunzhen Wu,
  • Jingya Yuan,
  • Like Shen,
  • Qinxue Li,
  • Zhuomeng Li,
  • Hongwei Cao,
  • Lin Zhu,
  • Dan Liu,
  • Yalu Sun,
  • Qianru Jia,
  • Huatao Chen,
  • Wubin Wang,
  • Jörg Kudla,
  • Wenhua Zhang,
  • Junyi Gai,
  • Qun Zhang

摘要

Chloride (Cl−) ions cause major damage to crops in saline soils. Understanding the key factors that influence Cl− uptake and translocation will aid the breeding of more salt-tolerant crops. Here, using genome-wide association study and transcriptomic analysis, we identified a NITRATE TRANSPORTER 1 (NRT1)/PEPTIDE TRANSPORTER family (NPF) protein, GmNPF7.5, as the dominant gene locus influencing Cl− homeostasis in soybean (Glycine max). A natural SNP variation resulted in two haplotypes (GmNPF7.5HapA and GmNPF7.5HapB), which was associated with Cl− content. GmNPF7.5HapA mediated Cl− or nitrate (NO3−) uptake in a pH-dependent manner and exhibited higher permeability for Cl− over NO3−. The suppression of GmNPF7.5HapA expression decreased Cl− accumulation and salt damage in plants, whereas its overexpression showed the opposite effects. The elite haplotype GmNPF7.5HapB diminished Cl− transport activity independently from NO3− permeability, thus enhancing soybean salt tolerance. Furthermore, the protein kinase GmPI4Kγ4 could phosphorylate GmNPF7.5, which repressed Cl− uptake without affecting NO3− permeability. Our findings define a regulatory mechanism for Cl− control under NaCl stress, providing a strategy for the improvement of salt tolerance in soybean plants.