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<p>Traditional chemical interventions regulate cellular processes but often affect non-target biomolecules. Precise and site-specific control is crucial for studying complex systems. Conventional laser-based methods offer high spatial precision and speed but rely on prior sample knowledge and do not apply to highly mobile targets. Real-time precision opto-control (RPOC) overcomes these limits using closed-loop feedback for automated and signal-determined real-time laser activation to regulate chemical processes in live biological samples. This review compares RPOC with other optical control techniques and explores its advancements, applications, and future directions.</p>

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Advances and future trends in real-time precision optical control of chemical processes in live cells

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年5月28日

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  • Chi Zhang,
  • Bin Dong,
  • Shivam Mahapatra,
  • Seohee Ma

摘要

Traditional chemical interventions regulate cellular processes but often affect non-target biomolecules. Precise and site-specific control is crucial for studying complex systems. Conventional laser-based methods offer high spatial precision and speed but rely on prior sample knowledge and do not apply to highly mobile targets. Real-time precision opto-control (RPOC) overcomes these limits using closed-loop feedback for automated and signal-determined real-time laser activation to regulate chemical processes in live biological samples. This review compares RPOC with other optical control techniques and explores its advancements, applications, and future directions.