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<p>The microbial valorization of glycerol, a major biodiesel byproduct, into high-value chemicals remains challenging at industrially competitive titers. Here we engineered <i>Corynebacterium glutamicum</i> for high-level 1,3-propanediol (1,3-PDO) production, validating each step via fed-batch fermentation. First, <i>C. glutamicum</i> ATCC 13032 was metabolically engineered to produce 138 g l<sup>−1</sup> 1,3-PDO from glucose–glycerol and 100.9 g l<sup>−1</sup> from glycerol alone. Key engineering strategies included establishing glycerol uptake and 1,3-PDO biosynthetic pathways, minimizing byproducts and optimizing fed-batch fermentation. We then transferred these strategies to a newly isolated strain, <i>C. glutamicum</i> SC97. Further engineering, including antibiotic-free plasmid addiction system and <i>sucCD</i> overexpression, enabled 141.5 g l<sup>−1</sup> 1,3-PDO at 2.95 g l<sup>−1</sup> h<sup>−1</sup> without antibiotics. Scalability was demonstrated at 30-l and 300-l pilot-scale fermentations, reaching 120.2 g l<sup>−1</sup> and 127.8 g l<sup>−1</sup> of 1,3-PDO, respectively. Techno-economic and life-cycle assessments support industrial feasibility and environmental impact, providing a robust blueprint for sustainable microbial 1,3-PDO production at scale.</p><p></p>

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High-titer, antibiotic-free, pilot-scale production of 1,3-propanediol by engineered Corynebacterium

  • 期刊论文

  • 发表日期：

    2026年5月12日

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  • Jae Sung Cho,
  • Cindy Pricilia Surya Prabowo,
  • Taehee Han,
  • Cheon Woo Moon,
  • Yoo-Sung Ko,
  • Changhee Cho,
  • Je Woong Kim,
  • Won Jun Kim,
  • Hyun Bae Bang,
  • Jae Eun Lee,
  • Minjung Ki,
  • Namjin Jang,
  • Sang Yup Lee

摘要

The microbial valorization of glycerol, a major biodiesel byproduct, into high-value chemicals remains challenging at industrially competitive titers. Here we engineered Corynebacterium glutamicum for high-level 1,3-propanediol (1,3-PDO) production, validating each step via fed-batch fermentation. First, C. glutamicum ATCC 13032 was metabolically engineered to produce 138 g l−1 1,3-PDO from glucose–glycerol and 100.9 g l−1 from glycerol alone. Key engineering strategies included establishing glycerol uptake and 1,3-PDO biosynthetic pathways, minimizing byproducts and optimizing fed-batch fermentation. We then transferred these strategies to a newly isolated strain, C. glutamicum SC97. Further engineering, including antibiotic-free plasmid addiction system and sucCD overexpression, enabled 141.5 g l−1 1,3-PDO at 2.95 g l−1 h−1 without antibiotics. Scalability was demonstrated at 30-l and 300-l pilot-scale fermentations, reaching 120.2 g l−1 and 127.8 g l−1 of 1,3-PDO, respectively. Techno-economic and life-cycle assessments support industrial feasibility and environmental impact, providing a robust blueprint for sustainable microbial 1,3-PDO production at scale.