Scholarlink

<p>Antimicrobial resistance is a global health threat, partly driven by the spread of extended-spectrum β-lactamase (ESBL)-producing bacteria. The use of β-lactam antibiotics in healthcare and agriculture contributes to this emergence. The β-lactam antibiotic cloxacillin, a narrow-spectrum antibiotic, is widely applied in dairy farms during dry cow treatment (DCT). Paralleling this use is an increasing incidence of ESBL producing gram-negative bacteria detected in the Dutch dairy sector. To investigate whether cloxacillin inadvertently affects the growth of <i>E. coli</i> and ESBL-producing <i>E. coli</i>, we measured the hydrolytic activity of purified β-lactamase enzymes, assessed their impact on the antibiotic susceptibility and growth kinetics of wild-type and recombinant <i>E. coli</i> and determined cloxacillin concentrations in colostrum. Our findings show that β-lactamases bind and hydrolyze cloxacillin, resulting in an increase in MIC when recombinantly expressed in hypersensitive <i>E. coli</i>. These results suggest that cloxacillin, although not clinically active against gram-negative bacteria, may influence the growth of β-lactamase-producing strains, warranting further investigations, specifically of the possible selective effect on ESBL-producing bacteria.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

β-lactamase-mediated hydrolysis of cloxacillin increases tolerance in Escherichia coli

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年6月23日

查看全文
  • Nora C. Brüchle,
  • Kees T. Veldman,
  • Quillan Dijkstra,
  • Michiel de Boer,
  • Eduardo de Freitas Costa,
  • Robbert van den Beld,
  • Steven Sietsma,
  • Milou G. M. van de Schans,
  • Nathaniel I. Martin,
  • Michael S. M. Brouwer

摘要

Antimicrobial resistance is a global health threat, partly driven by the spread of extended-spectrum β-lactamase (ESBL)-producing bacteria. The use of β-lactam antibiotics in healthcare and agriculture contributes to this emergence. The β-lactam antibiotic cloxacillin, a narrow-spectrum antibiotic, is widely applied in dairy farms during dry cow treatment (DCT). Paralleling this use is an increasing incidence of ESBL producing gram-negative bacteria detected in the Dutch dairy sector. To investigate whether cloxacillin inadvertently affects the growth of E. coli and ESBL-producing E. coli, we measured the hydrolytic activity of purified β-lactamase enzymes, assessed their impact on the antibiotic susceptibility and growth kinetics of wild-type and recombinant E. coli and determined cloxacillin concentrations in colostrum. Our findings show that β-lactamases bind and hydrolyze cloxacillin, resulting in an increase in MIC when recombinantly expressed in hypersensitive E. coli. These results suggest that cloxacillin, although not clinically active against gram-negative bacteria, may influence the growth of β-lactamase-producing strains, warranting further investigations, specifically of the possible selective effect on ESBL-producing bacteria.