Scholarlink

<p><i>Proteus mirabilis</i>, a Gram-negative bacterium renowned for its distinctive swarming motility, is a major causative agent of catheter-associated urinary tract infections (CAUTIs) and nosocomial complications. While advances in sequencing technologies have generated extensive genomic data, critical gaps persist in understanding the global population structure and evolutionary drivers of antimicrobial resistance in this pathogen. To address this knowledge gap, we performed a phylogenomic analysis of 1,142 <i>P. mirabilis</i> genomes spanning 34 countries and 16 ecological niches. Our investigation identified a dominant multidrug-resistant lineage (Cluster-1) carrying significantly elevated antimicrobial resistance gene burdens, including high-prevalence carbapenemase genes <i>bla</i><sub>KPC-2</sub> and <i>bla</i><sub>IMP-27</sub>. Bayesian evolutionary dating traced the most recent common ancestor of Cluster-1 to approximately 1910, with subsequent expansion linked to acquisition of the autotransporter gene <i>agn43</i> within the PmGRI1 genomic island. Notably, Cluster-1 diversified into two clinically significant subclades: a China-associated branch carrying <i>bla</i><sub>KPC-2</sub> and a USA-associated branch harboring <i>bla</i><sub>IMP-27</sub>. Functional characterization revealed that <i>agn43</i> deletion caused significant attenuation in biofilm formation, heat stress tolerance, and swarming motility. Our findings delineate the emergence of a globally disseminated <i>P. mirabilis</i> clone, highlighting the synergistic role of antimicrobial resistance and <i>agn43</i>-mediated adaptive traits in its epidemiological success.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Genomic survey uncovers the emergence of a multidrug-resistant dominant lineage in Proteus mirabilis populations

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年3月3日

查看全文
  • Tiejun Zhang,
  • Hongcheng Wei,
  • Zijing Ju,
  • Qin Wang,
  • Jinpeng Li,
  • Renqiao Wen,
  • Jie Wu,
  • Hongning Wang,
  • Changwei Lei

摘要

Proteus mirabilis, a Gram-negative bacterium renowned for its distinctive swarming motility, is a major causative agent of catheter-associated urinary tract infections (CAUTIs) and nosocomial complications. While advances in sequencing technologies have generated extensive genomic data, critical gaps persist in understanding the global population structure and evolutionary drivers of antimicrobial resistance in this pathogen. To address this knowledge gap, we performed a phylogenomic analysis of 1,142 P. mirabilis genomes spanning 34 countries and 16 ecological niches. Our investigation identified a dominant multidrug-resistant lineage (Cluster-1) carrying significantly elevated antimicrobial resistance gene burdens, including high-prevalence carbapenemase genes blaKPC-2 and blaIMP-27. Bayesian evolutionary dating traced the most recent common ancestor of Cluster-1 to approximately 1910, with subsequent expansion linked to acquisition of the autotransporter gene agn43 within the PmGRI1 genomic island. Notably, Cluster-1 diversified into two clinically significant subclades: a China-associated branch carrying blaKPC-2 and a USA-associated branch harboring blaIMP-27. Functional characterization revealed that agn43 deletion caused significant attenuation in biofilm formation, heat stress tolerance, and swarming motility. Our findings delineate the emergence of a globally disseminated P. mirabilis clone, highlighting the synergistic role of antimicrobial resistance and agn43-mediated adaptive traits in its epidemiological success.