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<p>End-stage organ failure requires the transplantation of a new organ. However, the number of patients awaiting donor organs exceeds the number of available organs. To address this organ shortage, solid organs, such as the kidneys, liver, heart and lungs, can be engineered based on decellularized human and non-human tissues. These decellularized scaffolds can then be recellularized with autologous or allogeneic cells and modified to ensure engraftment and function following transplantation. In this Review, we discuss the creation of decellularized neo-solid organs, including animal donor considerations, pre-decellularization processes, decellularization protocols, post-decellularization characterization, sterilization and storage conditions. We highlight various cell seeding and modification strategies and examine bioreactor culture conditions to grow functional solid organs. Finally, we outline mechanisms of transplant-recipient crosstalk and discuss challenges and opportunities for the clinical translation of engineered solid organs.</p>

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Ex vivo organ engineering using decellularized tissue scaffolds

  • 期刊论文

  • 发表日期：

    2025年6月16日

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  • Tarek Saleh,
  • Lorenzo Caciolli,
  • Giovanni Giuseppe Giobbe,
  • Paolo De Coppi

摘要

End-stage organ failure requires the transplantation of a new organ. However, the number of patients awaiting donor organs exceeds the number of available organs. To address this organ shortage, solid organs, such as the kidneys, liver, heart and lungs, can be engineered based on decellularized human and non-human tissues. These decellularized scaffolds can then be recellularized with autologous or allogeneic cells and modified to ensure engraftment and function following transplantation. In this Review, we discuss the creation of decellularized neo-solid organs, including animal donor considerations, pre-decellularization processes, decellularization protocols, post-decellularization characterization, sterilization and storage conditions. We highlight various cell seeding and modification strategies and examine bioreactor culture conditions to grow functional solid organs. Finally, we outline mechanisms of transplant-recipient crosstalk and discuss challenges and opportunities for the clinical translation of engineered solid organs.