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<p>Microbial consortia hold immense promise for wastewater treatment by harnessing metabolite exchange-based interspecies interactions to drive energy and matter flow. Yet, challenges in the coordinated modulation of transmembrane transport proteins and extracellular metabolite distribution limit cross-species interactions within consortia. Here we propose a mesospace-domain regulation strategy that leverages hydrogel-assembled mesoscale habitats to precisely modulate β-barrel membrane porins and locally enrich cross-fed molecules, thus remodelling interspecific cooperative metabolism for efficient wastewater treatment. Confining a carefully designed microbiota within mesospace enhances organic wastewater treatment for hexanoate production, achieving a 307.2% higher yield than unconfined systems. This improvement is attributed to mesospace-governed porin regulation and exometabolite enrichment, which reprogram interbacterial interactions from unidirectional electron transfer to bidirectional multimetabolite cross-feeding. This regulatory strategy is also applicable to other wastewater treatment systems, markedly enhancing succinic acid production, denitrification of low carbon-to-nitrogen ratio wastewater and removal of emerging contaminants. These findings illuminate how the mesospace domain orchestrates microbiota metabolism to boost bioconversion efficiency and selectivity for sustainable wastewater management.</p>

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Mesospace domain orchestrates microbial consortia by β-barrel porin modulation and local molecule enrichment for wastewater treatment

  • 期刊论文

  • 发表日期：

    2026年1月27日

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  • Chao Liu,
  • Yue Yin,
  • Xuemeng Zhang,
  • Wei-Min Wu,
  • Bingcai Pan,
  • Hongqiang Ren,
  • Qingran Zhang,
  • Han-Qing Yu,
  • Jinfeng Wang,
  • Mengru Zhu,
  • Yinguang Chen

摘要

Microbial consortia hold immense promise for wastewater treatment by harnessing metabolite exchange-based interspecies interactions to drive energy and matter flow. Yet, challenges in the coordinated modulation of transmembrane transport proteins and extracellular metabolite distribution limit cross-species interactions within consortia. Here we propose a mesospace-domain regulation strategy that leverages hydrogel-assembled mesoscale habitats to precisely modulate β-barrel membrane porins and locally enrich cross-fed molecules, thus remodelling interspecific cooperative metabolism for efficient wastewater treatment. Confining a carefully designed microbiota within mesospace enhances organic wastewater treatment for hexanoate production, achieving a 307.2% higher yield than unconfined systems. This improvement is attributed to mesospace-governed porin regulation and exometabolite enrichment, which reprogram interbacterial interactions from unidirectional electron transfer to bidirectional multimetabolite cross-feeding. This regulatory strategy is also applicable to other wastewater treatment systems, markedly enhancing succinic acid production, denitrification of low carbon-to-nitrogen ratio wastewater and removal of emerging contaminants. These findings illuminate how the mesospace domain orchestrates microbiota metabolism to boost bioconversion efficiency and selectivity for sustainable wastewater management.