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<p>Wastewater surveillance can track trends in multiple pathogens simultaneously by leveraging efficient laboratory processing. In Switzerland, wastewater surveillance of four respiratory pathogens is conducted at 14 locations representing 2.3 million people. Trends in respiratory diseases are tracked using a six-plex digital PCR assay targeting influenza A, influenza B, respiratory syncytial virus and SARS-CoV-2 N1 and N2 regions and murine hepatitis virus for recovery efficiency control. Wastewater data were integrated with disease data from two reporting systems, and comparisons from July 2023 to July 2024 showed strong agreement for most targets. Lower correspondence for influenza B highlighted challenges in tracking disease dynamics during seasons without pronounced outbreaks. Wastewater monitoring further revealed that targeting N1 or N2 led to divergent estimates of SARS-CoV-2 loads, highlighting the impact of mutations in assay target regions. The study emphasizes the importance of an integrated wastewater monitoring programme as a complementary tool for public health surveillance, demonstrating clear concordance with clinical data.</p>

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A six-plex digital PCR assay for monitoring respiratory viruses in wastewater

  • 期刊论文

  • 发表日期：

    2025年9月22日

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  • Melissa Pitton,
  • Rachel E. McLeod,
  • Lea Caduff,
  • Ayazhan Dauletova,
  • Jolinda de Korne-Elenbaas,
  • Charles Gan,
  • Camille Hablützel,
  • Aurélie Holschneider,
  • Seju Kang,
  • Guy Loustalot,
  • Patrick Schmidhalter,
  • Linda Schneider,
  • Anna Wettlauffer,
  • Daniela Yordanova,
  • Timothy R. Julian,
  • Christoph Ort

摘要

Wastewater surveillance can track trends in multiple pathogens simultaneously by leveraging efficient laboratory processing. In Switzerland, wastewater surveillance of four respiratory pathogens is conducted at 14 locations representing 2.3 million people. Trends in respiratory diseases are tracked using a six-plex digital PCR assay targeting influenza A, influenza B, respiratory syncytial virus and SARS-CoV-2 N1 and N2 regions and murine hepatitis virus for recovery efficiency control. Wastewater data were integrated with disease data from two reporting systems, and comparisons from July 2023 to July 2024 showed strong agreement for most targets. Lower correspondence for influenza B highlighted challenges in tracking disease dynamics during seasons without pronounced outbreaks. Wastewater monitoring further revealed that targeting N1 or N2 led to divergent estimates of SARS-CoV-2 loads, highlighting the impact of mutations in assay target regions. The study emphasizes the importance of an integrated wastewater monitoring programme as a complementary tool for public health surveillance, demonstrating clear concordance with clinical data.