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<p>We present an open-source code for 3D super-resolution ultrasound imaging. Open-3DULM was applied to transcranial imaging in habituated awake mice. Comparative analysis reveals that isoflurane anesthesia induces significant vasodilation and increased cerebral blood flow (CBF) in veins, while only a subset of arteries exhibits these effects compared to the awake state. This method could serve as a reference for developing new types of 3D vascular quantifications, particularly emphasizing the importance of awake and 3D imaging for accurate cerebral blood flow measurement in neuroscience research.</p>

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3D transcranial ultrasound localization microscopy in awake mice: protocol and open-source pipeline

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年6月6日

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  • Georges Chabouh,
  • Louise Denis,
  • Myriam Abioui-Mourgues,
  • Jean-Baptiste Deloges,
  • Jacques Battaglia,
  • Arthur Chavignon,
  • Baptiste Heiles,
  • Basile Pradier,
  • Sylvain Bodard,
  • Denis Vivien,
  • Cyrille Orset,
  • Olivier Couture

摘要

We present an open-source code for 3D super-resolution ultrasound imaging. Open-3DULM was applied to transcranial imaging in habituated awake mice. Comparative analysis reveals that isoflurane anesthesia induces significant vasodilation and increased cerebral blood flow (CBF) in veins, while only a subset of arteries exhibits these effects compared to the awake state. This method could serve as a reference for developing new types of 3D vascular quantifications, particularly emphasizing the importance of awake and 3D imaging for accurate cerebral blood flow measurement in neuroscience research.