<p>Lutein is commonly used as a supplement for ocular health. However, commercial lutein is mainly extracted from marigold flowers, an inefficient process that includes secondary metabolites. Here, building on our previous research on lutein synthesis in engineered <i>Escherichia coli</i>, we report the development of a microbial platform for efficient lutein production using metabolically engineered <i>Corynebacterium glutamicum</i>. We engineered <i>C.</i> <i>glutamicum</i> to produce lycopene and introduced the lutein biosynthesis pathway. Then, we substantially enhanced lutein production by optimizing the haem pathway, engineering the P450 reductase and utilizing an optimal electron-channelling scaffold system to improve electron transfer reactions, addressing the rate-limiting steps in lutein synthesis. This approach achieved a substantial increase in lutein production, reaching a concentration of 1.78 g l⁻¹, with a content of 19.51 mg per gram dry cell weight and a productivity of 32.88 mg l⁻¹ h⁻¹ in fed-batch fermentation. This approach allows gram-per-litre-scale microbial production of lutein.</p><p></p>