mRNA m⁶A detection

N⁶-methyladenosine (m⁶A) is the most prevalent internal mRNA modification. Recent research has highlighted its role as a key regulator of gene expression, influencing cellular processes and determining cell fate. Advances in techniques for global mapping of m⁶A, the discovery of m⁶A demethylases that enhance its dynamic properties and the identification of reader proteins that interact with m⁶A have substantially propelled this field forward. This Primer outlines the available tools for detecting and mapping m⁶A, discusses the strengths and limitations of each method and offers guidance on selecting the most suitable approach. Identifying and detecting m⁶A lays the groundwork for functional studies that address important biological and medical questions.