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<p>The presence of microbiota in human tumors has been reported widely based on bioinformatic analyses of DNA sequencing datasets; however, the source of microbial sequences in atypical anatomical sites is challenging to validate, as these could derive from sampling, storage, handling and processing of samples, similar to what has been described in studies of ancient DNA. Contamination of microbial reference genomes can also be a source of microbial signals, causing misclassification of human reads. Here, we overview the required quality controls and validation approaches and summarize optimal practices to improve the rigor and standards of tumor microbiome studies.</p>

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Setting higher standards for reports of microbial species in human cancers

  • 期刊论文

  • 发表日期：

    2026年2月19日

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  • Steven L. Salzberg,
  • Minghao Chia,
  • Alfred Tay,
  • Noel F.C.C. de Miranda,
  • Vincent Smit,
  • Jelle Wesseling,
  • Rafael Irizarry,
  • Barry Marshall,
  • Eske Willerslev,
  • Jacques Neefjes,
  • Niranjan Nagarajan

摘要

The presence of microbiota in human tumors has been reported widely based on bioinformatic analyses of DNA sequencing datasets; however, the source of microbial sequences in atypical anatomical sites is challenging to validate, as these could derive from sampling, storage, handling and processing of samples, similar to what has been described in studies of ancient DNA. Contamination of microbial reference genomes can also be a source of microbial signals, causing misclassification of human reads. Here, we overview the required quality controls and validation approaches and summarize optimal practices to improve the rigor and standards of tumor microbiome studies.