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<p>The constant expansion of the field of metabolic research has led to more nuanced and sophisticated understanding of the complex mechanisms that underlie metabolic functions and diseases. Collaborations with scientists of various fields such as neuroscience, immunology and drug discovery have further enhanced the ability to probe the role of metabolism in physiological processes. However, many behaviours, endocrine and biochemical processes, and the expression of genes, proteins and metabolites have daily ~24-h biological rhythms and thus peak only at specific times of the day. This daily variation can lead to incorrect interpretations, lack of reproducibility across laboratories and challenges in translating preclinical studies to humans. In this Review, we discuss the biological, environmental and experimental factors affecting circadian rhythms in rodents, which can in turn alter their metabolic pathways and the outcomes of experiments. We recommend that these variables be duly considered and suggest best practices for designing, analysing and reporting metabolic experiments in a circadian context.</p>

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The time is now: accounting for time-of-day effects to improve reproducibility and translation of metabolism research

  • 期刊论文

  • 发表日期：

    2025年3月17日

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  • Shaunak Deota,
  • Julie S. Pendergast,
  • Ullas Kolthur-Seetharam,
  • Karyn A. Esser,
  • Frédéric Gachon,
  • Gad Asher,
  • Charna Dibner,
  • Salvador Aznar Benitah,
  • Carolina Escobar,
  • Deborah M. Muoio,
  • Eric Erquan Zhang,
  • Gökhan S. Hotamışlıgil,
  • Joseph Bass,
  • Joseph S. Takahashi,
  • Joshua D. Rabinowitz,
  • Katja A. Lamia,
  • Rafael de Cabo,
  • Shingo Kajimura,
  • Valter D. Longo,
  • Ying Xu,
  • Mitchell A. Lazar,
  • Eric Verdin,
  • Juleen R. Zierath,
  • Johan Auwerx,
  • Daniel J. Drucker,
  • Satchidananda Panda

摘要

The constant expansion of the field of metabolic research has led to more nuanced and sophisticated understanding of the complex mechanisms that underlie metabolic functions and diseases. Collaborations with scientists of various fields such as neuroscience, immunology and drug discovery have further enhanced the ability to probe the role of metabolism in physiological processes. However, many behaviours, endocrine and biochemical processes, and the expression of genes, proteins and metabolites have daily ~24-h biological rhythms and thus peak only at specific times of the day. This daily variation can lead to incorrect interpretations, lack of reproducibility across laboratories and challenges in translating preclinical studies to humans. In this Review, we discuss the biological, environmental and experimental factors affecting circadian rhythms in rodents, which can in turn alter their metabolic pathways and the outcomes of experiments. We recommend that these variables be duly considered and suggest best practices for designing, analysing and reporting metabolic experiments in a circadian context.