Scholarlink

<p>Biomolecular condensates formed by intrinsically disordered regions of proteins are primarily stabilised by amino acid “stickers” that crosslink polypeptide chains. While aromatic and positively charged residues both act as “stickers”, their interaction mechanisms differ. Quantifying these differences is essential since they determine the fate of multicomponent mixtures. Here, we show that the two main positively charged stickers, arginine (Arg) and lysine (Lys), exhibit a consistent hierarchy where Arg promotes phase separation more effectively than Lys. Using molecular dynamics simulations and quantum chemical calculations, we find that, unlike aromatic residues, Arg and Lys consistently maintain this hierarchy across diverse molecular environments. While cation-<i>π</i> interactions are important, the primary factor driving this difference is the higher dehydration penalty of Lys. In contrast, the preferred aromatic partner for these cations depends on the dielectric environment. These results explain at a molecular level the distinct contributions of Arg and Lys to condensate stability.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Molecular determinants of arginine versus lysine cation–π interactions in biomolecular condensates

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年5月26日

查看全文
  • Lydia Armentia,
  • Xabier López,
  • David De Sancho

摘要

Biomolecular condensates formed by intrinsically disordered regions of proteins are primarily stabilised by amino acid “stickers” that crosslink polypeptide chains. While aromatic and positively charged residues both act as “stickers”, their interaction mechanisms differ. Quantifying these differences is essential since they determine the fate of multicomponent mixtures. Here, we show that the two main positively charged stickers, arginine (Arg) and lysine (Lys), exhibit a consistent hierarchy where Arg promotes phase separation more effectively than Lys. Using molecular dynamics simulations and quantum chemical calculations, we find that, unlike aromatic residues, Arg and Lys consistently maintain this hierarchy across diverse molecular environments. While cation-π interactions are important, the primary factor driving this difference is the higher dehydration penalty of Lys. In contrast, the preferred aromatic partner for these cations depends on the dielectric environment. These results explain at a molecular level the distinct contributions of Arg and Lys to condensate stability.