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<p>DNAzymes conventionally require dissolved metal ions for catalytic functions. Herein, we report that metal surfaces directly activate a self-cleaving DNAzyme (PL) at solid-liquid interfaces. PL exhibits activities on copper, vanadium and tantalum surfaces, within a minimal reaction system comprising only the metal surface, PL and double-distilled water. This interfacial activation is highly material-specific, showing complete absence of activity on plastics, glass or wood etc. Mechanistic studies reveal that dissolved oxygen could react with metal surfaces to generate superoxide anions, which serve as triggers for DNA-cleavage. The reaction shows modulatable characteristics, with inhibition by ethylenediaminetetraacetic acid, catalase, nitroblue tetrazolium and cytochrome <i>c</i>, versus enhancement by vitamin C, glutathione and catechol. Furthermore, metal surface-mediated activation was also observed in F-8, Ag10c and I-R3 DNAzymes, indicating that this phenomenon is not an isolated occurrence. This work establishes macroscopic metals as DNAzyme’s cofactors, extending DNAzyme catalysis beyond conventional homogeneous systems to heterogeneous interfacial environments.</p><p></p>

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Metal surface-triggered DNAzyme catalysis for efficient DNA cleavage

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年1月19日

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  • Fangning Jiang,
  • Yan Dong,
  • Wenqian Yu,
  • Huiyu Tian,
  • Longping Yang,
  • Ziyi Jia,
  • Yongjie Sheng,
  • Dayong Si,
  • Jiacui Xu,
  • Dazhi Jiang

摘要

DNAzymes conventionally require dissolved metal ions for catalytic functions. Herein, we report that metal surfaces directly activate a self-cleaving DNAzyme (PL) at solid-liquid interfaces. PL exhibits activities on copper, vanadium and tantalum surfaces, within a minimal reaction system comprising only the metal surface, PL and double-distilled water. This interfacial activation is highly material-specific, showing complete absence of activity on plastics, glass or wood etc. Mechanistic studies reveal that dissolved oxygen could react with metal surfaces to generate superoxide anions, which serve as triggers for DNA-cleavage. The reaction shows modulatable characteristics, with inhibition by ethylenediaminetetraacetic acid, catalase, nitroblue tetrazolium and cytochrome c, versus enhancement by vitamin C, glutathione and catechol. Furthermore, metal surface-mediated activation was also observed in F-8, Ag10c and I-R3 DNAzymes, indicating that this phenomenon is not an isolated occurrence. This work establishes macroscopic metals as DNAzyme’s cofactors, extending DNAzyme catalysis beyond conventional homogeneous systems to heterogeneous interfacial environments.